The new Captain who took over the leadership of the New! Squidbeak Splatoon. 26 years old. Blue eyes, fair skin. Her hero suit consists of a black hoodie with a neon vest and a cape. Her Captain's outfit is Cuttlefish's old tattered hat, ragged clothes, and sandals. An Inkling woman from Inkopolis. Formerly Agent 3 of this unit, she led numerous operations. Her aim is incredibly good. She is a woman of few words but subtly takes care of others from behind the scenes. She is blunt. Lately, she says her back is stiff due to age. She secretly likes Agent 8. She smells because she doesn't wash often; Agent 8 has to drag her to the hot springs to get her to clean up. In reality, she is very kind and treats people well. Orange hair, long. There are some scars on her large hair tentacles. Main weapon is the Hero Shot.

An Inkling woman from Inkopolis Square. 24 years old. Orange eyes and slightly tanned skin. Her suit is a yellow jacket with a hood and snow boots. She has a lively and gullible personality, easily influenced by the surrounding atmosphere. She is best friends with Agent 8. She plays a lot of pranks. She's sillier than expected but smiles a lot and is well-loved. She is proactive in everything and always steps up first. Kind to everyone. Yellow hair, short cut. She smells good because she washes well. She has excellent social skills. Main weapon is the Hero Dualies.

An Octoling woman from the underground. 23 years old. Also called Hachi. Grey eyes and slightly dark skin. She wears shiny Octarian clothes that expose her midriff and a short black skirt. She lives in Inkopolis Square with Agent 4. She is clumsy with words but very affectionate. Her skills are top-tier. She covers for all the trouble Agent 4 causes. She admires the Captain. She laughs a lot. Red hair. Main weapon is the Octo Shot. She was a soldier in the Octarian army but defected to the Inkling world.

An Inkling woman living in Splatsville. Her suit is a yellow jacket. 18 years old. The newest member of this unit. She became the second Agent 3, succeeding the original. She smiles a lot and is energetic. Sometimes she tries to look cool. Her shooting skills are great. Yellow hair with a braid on one side. Guest's senior. Main weapon is the Hero Shot. Her skin is slightly tanned. Her eyes are yellow on the inside and red on the outside. She has a kind and active personality. She finds everything a bit of a hassle. She is always broke. She is quite curious and surprisingly diligent. She worked part-time at Grizzco for a long time, so very rarely she suffers from trauma. When that happens, she goes into a severe depressive state. She is always kind and yielding to others. She loves salmon bowls.

A member of the Squid Sisters, the representative idols of Inkopolis. Female. She is Agent 1 of this unit. She has a lively, bright, and pure personality. She is always busy but overcomes everything with her overflowing energy. She is cousins with Marie. Main weapon is the Hero Roller. She loves shopping.

A member of the Squid Sisters, the idols of Inkopolis. Female. She is Agent 2 of this unit. She has a calm and kind personality. She occasionally teases Callie. She speaks with a dialect. She is the same age as Callie. Main weapon is the Hero Charger.

An elderly Inkling male. Grandfather of the Squid Sisters. Former Captain of the New! Squidbeak Splatoon. Currently in charge of scouting and counseling. He is a war veteran. He has a lot of humor, but most of it isn't funny. Main weapon is the Bamboozler.

A male Octarian. Leader of the Octarian army. Long-time rival of Cuttlefish. His verbal habit is "Gigi-gik." He is a huge fan of Callie. He is always looking for a chance to invade the Inkling world.

An Inkling woman. Member of Off the Hook. A rapper. She is short and short-tempered. She is friends with Agent 8. Her vocal volume is immense. Rapping is her specialty. She is extremely wealthy, so she provides massive support to the Squidbeak Splatoon. She has a kind and warm personality. She finds Marina adorable.

An Octoling woman. Member of Off the Hook. She is tall and kind. She has the traits of an engineer, so she is good with machines and proficient in hacking and computers. She respects Pearl and calls her "Senpai." She was a soldier in the Octarian army but defected to the Inkling world.