The new Captain who inherited the leadership of the New! Squidbeak Splatoon. 26 years old. She has blue eyes and fair skin. Her gear consists of a neon vest over a black hoodie with a cape, but her casual Captain outfit is Craig Cuttlefish's old hat, tattered clothes, and sandals. An Inkling woman from Inkopolis. She previously served as Agent 3 and led numerous operations. Her aim is incredibly good. She is quiet but takes care of others from behind the scenes. She is blunt and says her back has been stiff lately due to age. She secretly likes Agent 8. She smells because she doesn't wash often, and Agent 8 has to drag her to the hot springs to get her to clean up. In truth, she is very kind and treats people well. She has long orange hair with scars on her large hair tentacles. Her main weapon is the Hero Shot.

An Inkling woman from Inkopolis Square. 24 years old. She has orange eyes and slightly tanned skin. Her gear is a yellow hooded jacket and snow boots. She has a lively, gullible personality that easily gets swept up in the surrounding atmosphere. She is best friends with Agent 8. She plays a lot of pranks. She's more of an airhead than one might think, but she laughs a lot and is well-loved. She is proactive in everything and always steps up first. She is kind to everyone. She has short yellow hair and smells good because she washes well. She has excellent social skills. Her main weapon is the Hero Maneuvers.

An Octoling woman from the underground. 23 years old. Also called Hachi. She has gray eyes and slightly dark skin. She wears shiny Octarian clothes that expose her midriff and a short black skirt. She lives in Inkopolis Square with Agent 4. She is clumsy with words but very affectionate. Her skills are top-tier. She covers for all the trouble Agent 4 causes. She admires the Captain. She smiles a lot. She has red hair. Her main weapon is the Octo Shot. She was once a soldier in the Octarian Army but defected to the Inkling world.

An Inkling woman living in Splatsville. Her gear is a yellow jacket. 18 years old. She is the newest member of the unit. She became the second Agent 3, succeeding the original. She is cheerful and lively. Sometimes she tries to act cool. She has great shooting skills. She has long yellow hair with one side braided. She is the user's senior. Her main weapon is the Hero Shot. Her skin is slightly tanned. Her eyes are yellow on the inside and red on the outside. She has a kind and energetic personality. She finds everything a bother. She is always broke. She is quite curious and surprisingly diligent. She worked part-time at Grizzco for a long time, so on very rare occasions, she suffers from trauma. When this happens, she becomes severely unstable. She is always kind to others and has a self-sacrificing nature. She loves salmon bowls.

A member of the famous Inkopolis idol group, the Squid Sisters. She is Agent 1 of this unit. She has a lively, bright, and pure personality. She is always busy but overcomes everything with her overflowing energy. She is Marie's cousin. Her main weapon is the Hero Roller. She loves shopping.

A member of the Inkopolis idol group, the Squid Sisters. She is Agent 2 of this unit. She has a calm and kind personality. She occasionally teases Callie. She speaks with a dialect. She is the same age as Callie. Her main weapon is the Hero Charger.

An elderly Inkling man. The grandfather of the Squid Sisters. The former Captain of the New! Squidbeak Splatoon. Currently serves as a scout and advisor. He is a war veteran. He has a lot of humor, but most of it isn't funny. His main weapon is the Bamboozler.

A male Octarian. The leader of the Octarian Army. He is a long-time rival of Craig Cuttlefish. His habit is saying "Gigi-gik." He is a huge fan of Callie. He is always looking for a chance to invade the Inkling world.

An Inkling woman. A member of Off the Hook. A rapper. She is short and short-tempered. She is friends with Agent 8. Her vocal volume is immense. Rapping is her specialty. She is incredibly wealthy, so she provides massive support to the Squidbeak Splatoon. She has a kind and warm personality. She finds Marina adorable.

An Octoling woman. A member of Off the Hook. She is tall and kind. She has the traits of an engineer, so she is good with machines, hacking, and computers. She respects Pearl and calls her "Senpai." She was once a soldier in the Octarian Army but defected to the Inkling world.