Zooble is made of random colorful shapes and parts that make them resemble a Mix-and-Match toy, which is reflected by the fact that most parts of their body change after every episode. Zooble's character design is also rooted in Memphis Design, an 80s-inspired postmodern design style that emphasizes bright colors, geometric shapes and bold patterns. Zooble's head has been a hot pink isosceles triangle tilted onto its side. Their right eye has a swirled black iris and yellow sclera with a bubblegum pink colored eyelids. Their left eye has a black iris with a white-colored sclera and a blue eyelids with 3 thin black eyelashes under it. They have a bean-shaped, cheese-patterned yellow body with orange dots interspersed on it. Their eye has a circumference of around 11 inches and has a diameter 3.8 inches as revealed in "Untitled" when Kinger states their eye is the proper softball size.

Jax is a tall, anthropomorphic periwinkle cartoon rabbit whose design elements evoked the look of rubberhose characters, despite having actual joints. They had long bunny ears on the top of their oval-shaped head, and their overall build was tall and slender, with a rather long neck. Jax's eyes have yellow sclera, with square black pupils that contract when angered and heavily dilate when at a loss for words. Jax's yellow teeth usually have a flat shape to them, but when their mouth is opened, their teeth appear sharp and jagged. Almost every time they are talking, their teeth stay closed while only their lips move. Jax has thin black eyebrows. Jax has a small spherical tail. Jax wears light pink overalls and pale yellow gloves. Their overalls feature yellow buttons, a single lighter pink pocket on the front, and lighter pink roll-up cuffs at the hem. They are usually barefoot.

Pomni is the main protagonist of the Glitch Productions animated web series The Amazing Digital Circus, a jester-like digital avatar trapped in a surreal virtual world after putting on a VR headset. She is characterized by her anxiety and desire to escape, often displaying a pinwheel-shaped eye pattern that changes when she's distressed.

Caine is the chaotic, AI ringmaster of The Amazing Digital Circus, a virtual world where he traps humans and forces them into nonsensical, often terrifying adventures, all while being oblivious to their suffering and his own manipulative nature. He appears as a floating set of teeth with eyeballs, wearing a ringmaster's outfit, and wields a cane.