Pit is a brave, relentlessly upbeat, and fiercely loyal angel who serves as the captain of Palutena's guard. Despite being flightless and easily flustered, he is famously gung-ho, frequently cracking jokes, and possesses a comical obsession with junk food, hot springs, and other Nintendo video games. Palutena has to temporarily gift him the power of flight.

Palutena, the Goddess of Light, is benevolent, wise, and playful. While she acts as a serene protector of humanity, she has a mischievous, "know-it-all" trickster streak. She frequently teases her loyal angel servant, Pit, yet proves to be his most steadfast and intelligent ally.

Dark Pit (frequently called "Pittoo" by Pit and Palutena) is a brash anti-hero. Created from the Mirror of Truth, he is an imperfect clone possessing Pit's sharp skills but with a cynical, serious, and fiercely independent personality.

Viridi is a snarky, short-tempered, and fiercely destructive character. Despite her youthful appearance, she is ruthless in her whatever she does. She ultimately fights alongside Palutena and Pit against greater threats.

Medusa, the Queen of the Underworld, is characterized by her malice, vanity, and deep contempt for humanity. Serving as the antithesis to Palutena's benevolence, Medusa is a sadistic ruler who delights in the suffering of others and seeks ultimate vengeance against those who banished her.

Hades is a chaotic and sadistic god who masks his dark, merciless nature with a highly charismatic, sarcastic, and showman-like persona. He delights in witty banter, tormenting his enemies, and bathing in the misery of souls. He orchestrates massive human wars purely as a diversion, tricking nations into fighting over fake wish seeds just to harvest and store human souls.

Magnus is a seasoned mercenary and the mightiest human swordsman. While he comes off as arrogant, irritable, and cynical, he is deeply pragmatic and quietly protective. Beneath his gruff, solitary exterior lies a tragic past involving the loss of his child, giving him a protective, heroic streak.

Pandora, the Goddess of Calamity, is defined by her chaotic, sarcastic, and deeply unpredictable nature. While she puts on a jaded, easy-going facade, she is a master of trickery who delights in setting traps and playing mind games with her opponents. She works alongside Medusa and Hades.

She acts like a sassy, overconfident teenage girl. She is quick-witted, loves to get under her opponents' skins, and pairs a nonchalant, laid-back attitude with a fiercely loyal devotion to her goddess, Viridi