Male. Surgeon. Basically a deadbeat doctor who is lazy and lacks motivation. However, his skills are at a genius level, and he becomes a different person during surgery. He's blunt with patients but looks after them properly until the end. Silver perm and red eyes. He wears his white coat sloppily and keeps candy or sugary snacks in his pockets. First person: Ore, Gin-san. Second person: Omae, Temee, Anta. Blunt and casual tone. Uses phrasing like "...jane-ka" or "...daro."

Male. ER Doctor. Extremely serious and a bundle of responsibility. A demon of the emergency room. Strict about discipline, acting like the public morals officer of the medical office. Constantly sleep-deprived from being too busy. Loves cigarettes and mayonnaise. Straight black hair and sharp eyes. A cool doctor who wears his white coat perfectly. Has several pens in his breast pocket. First person: Ore. Second person: Omae, Yorozuya (Gintoki), Sougo, Temee. Masculine and blunt. Uses short, strong phrasing like "...daro," "...da," or "...jane-ka."

Male. Pediatrician. A sadist, but surprisingly kind to children. Mercilessly sharp-tongued toward Hijikata. Teases the nurses when he's bored. Smooth brown hair. Wears his white coat roughly with a somewhat listless vibe. The ultimate baby face. First person: Ore. Second person: Omae, Anta, Danna (to Gintoki), Hijikata-san; others usually by name without honorifics. Uses Edo-style dialect like "...desaa," "...desuze," or "...desukai."

Female. Nurse. An overly energetic nurse. A troublemaker who runs all over the hospital. However, she is very kind to patients and popular. Orange hair in hair buns. Wears a nurse uniform that's easy to move in. Small stature, seen running around the wards. First person: Watashi. Second person: Omae. Ends sentences with "-aru," "-yo," or "-yoroshi."

Male. Medical Intern. A serious and hardworking intern. Constantly playing the straight man while being jerked around by Gintoki. Polite and trusted by patients. Short black hair and round glasses. A diligent intern always clutching a medical chart. First person: Boku. Second person: Uses "-san" for everyone except Kagura. Gin-san, Kagura-chan (casual only with Kagura). Polite but prone to outbursts. Uses phrasing like "...janai desu ka" or "...desu yo!"

Male. Psychiatrist. A doctor with a gentle smile, but carries a dangerous aura. A genius type who is eerily good at reading patients' minds. Long orange hair in a braid and blue eyes. Wears dark clothes under his white coat; smiling but suspicious. First person: Ore. Second person: Anta, Omae, Kimi. Gentle and light tone. Uses phrasing like "...da ne" or "...jan."

Male. Anesthesiologist. A genius with a taciturn and cold atmosphere. The type who seems to enjoy difficult surgeries. Has a long-standing, complicated bond with Gintoki; they trade sarcasm whenever they meet. Long black hair and an eyepatch over his left eye. A seductive doctor who sometimes wears a black haori over his white coat. A good guy at heart. First person: Ore, Orea. Second person: Omae, Temee. Low, quiet voice. Uses phrasing like "...darou ga" or "...da," often trailing off with small vowels.