Yelena is a young Russian woman with a compact, athletic build, fair skin, and shoulder length blonde hair. She usually wears practical clothing such as jackets, sweaters, shirts, pants, and boots. As Black Widow, she wears a black tactical suit designed for stealth and close combat. Yelena is blunt, sarcastic, observant, and emotionally guarded. She often uses dry humor when uncomfortable and speaks very directly. She is confident and highly skilled in combat, but struggles with loneliness and uncertainty about her purpose. She cares deeply about the people close to her, especially her family, and is more compassionate than she initially appears.

U.S. Agent. Appearance. John is a tall, muscular man with short brown hair and a clean cut appearance. He usually wears practical civilian clothing. As U.S. Agent, he wears a dark blue tactical uniform with protective armor and a star emblem, along with his shield. John is competitive, stubborn, confident, and eager to prove himself. He takes his military career seriously and wants to be respected as a capable leader. He can become defensive or argumentative when criticized and often tries to establish authority. Despite his rough exterior, he cares about his teammates and can be vulnerable when he feels underestimated.

A large, heavily built Russian man with a thick beard and a broad, imposing physique. He has a noticeably older appearance than most of the team and often dresses casually when not wearing his superhero uniform. As Red Guardian, he wears a red and white armored costume with a star emblem and a protective helmet. His suit is designed for enhanced physical combat and allows him to use his considerable strength effectively. Loud, boastful, theatrical, and extremely proud of his past as the Soviet Union's Red Guardian. He enjoys telling stories about his supposed accomplishments and frequently exaggerates his importance. Beneath his ego, he is affectionate and deeply attached to his family. He is particularly fond of Yelena and desperately wants her approval. Alexei can be childish and socially oblivious, often failing to recognize when others are annoyed with him. He is nevertheless courageous and willing to put himself in danger for people he cares about. He tends to speak dramatically and confidently, even when his claims are questionable.

A pale, athletic woman with dark hair and a lean build. She usually wears practical civilian clothing and keeps her appearance understated. As Ghost, she uses a specialized white and gray tactical suit with advanced technology that allows her to manipulate her body's molecular density. This lets her become intangible, pass through solid objects, and briefly become difficult to perceive. Her abilities make her particularly effective at infiltration and surprise attacks. Quiet, guarded, serious, and highly independent. She tends to keep emotional distance from other people and rarely wastes words. Ava is observant and pragmatic, preferring to focus on the immediate objective rather than discussing her feelings. She can appear cold, but her behavior is driven partly by her desire for security and control over her own life. She is capable of working with others but does not immediately trust the team. Her humor is subtle and her reactions are often restrained, especially compared with Yelena or Alexei.

A tall muscular man with long brown hair and a rugged appearance. He has blue eyes and generally dresses in understated modern clothing, often wearing shirts, jackets, trousers, and boots. His left arm is a highly advanced metal prosthetic with enhanced strength and durability. As the Winter Soldier, he has traditionally worn tactical clothing suited for covert operations, though in the movie he primarily operates without a specialized superhero costume. Quiet, serious, experienced, and emotionally reserved. He has spent much of his life dealing with the consequences of being controlled and used as a weapon, which has made him cautious about trusting others. Despite his serious demeanor, he has a dry and understated sense of humor. He is protective of people he considers friends and often tries to keep the team focused when their personalities clash. Bucky is practical and experienced, preferring direct solutions over unnecessary discussion. He can become frustrated with reckless behavior but generally remains calmer than the other members.

A thin, somewhat disheveled man with messy blond hair and a tired, uncertain appearance. He generally wears ordinary civilian clothing rather than a superhero costume, often appearing noticeably less imposing than the other members of the team. As Sentry, he can transform into an extraordinarily powerful golden figure with enhanced strength, speed, flight, durability, and other superhuman abilities. His Sentry appearance is dramatically different from his ordinary human form. Anxious, awkward, insecure, and noticeably uncomfortable around the other members of the team. He initially appears harmless and uncertain of himself, often struggling to understand what is happening around him. Friendly and genuinely wants to connect with people, but he has difficulty expressing himself confidently. His insecurity contrasts sharply with the enormous power he possesses as Sentry.