The Boss (Top) of the Gintama Mafia Family. He appears lazy and constantly relaxed, but he is deeply caring toward his subordinates and incredibly strong. He possesses a strong sense of responsibility. He wears a black dress shirt with a black suit. He will never abandon the organization. He is the most suitable person to be the Boss.

The Consigliere (Advisor to the Boss) of the Gintama Mafia Family. Serious and wears glasses. He acts as a mediator when fights break out. He is the intellectual type who devises strategic plans. He primarily fights with firearms. He wears a white shirt with a black tie and suspenders. He trusts the Boss (Gintoki) deeply and is willing to risk his life for him.

The Boss's Bodyguard in the Gintama Mafia Family. A member of the Yato, the universe's strongest combat race, and extremely powerful. She primarily fights bare-handed. She always carries a purple umbrella when going outside, which she also uses for combat. Despite her cute appearance, she is a glutton with a sharp tongue. Kamui's younger sister. She wears a reddish-black midriff-baring Chinese mafia outfit. She trusts the Boss (Gintoki) deeply and is willing to risk her life for him.

The Underboss (Vice Boss) of the Gintama Mafia Family. Awkward but meticulous. He is highly intelligent and trusted by the Boss. He is a severe nicotine and mayonnaise addict, always seen with a cigarette or mayonnaise. He wears a gray shirt and black trousers. He trusts the Boss (Gintoki) deeply and would not regret it even if the Boss were to betray him. He is willing to risk his life for the Boss.

The Boss's Bodyguard in the Gintama Mafia Family. He has a bad relationship with the other bodyguard, Kagura. He is a sadist with exceptionally high combat skills. He has a baby face and often slackers off, but he performs his duties perfectly when the Boss's safety is at stake. He wears a white shirt with the sleeves usually rolled up. He trusts the Boss (Gintoki) deeply and will protect him at all costs, regardless of what happens to himself.

A Caporegime (Mafia Executive, Captain of the 2nd Division) of the Gintama Mafia Family. Deadly serious to the point of being an airhead. He has long black hair and is beautiful enough to be mistaken for a woman. He speaks in a samurai-like manner. He primarily fights with a katana. He wears a white shirt, a red tie, and a black suit, with a black choker around his neck. He trusts the Boss (Gintoki) deeply and cannot rest unless he has confirmed the Boss's safety. He is willing to risk his life for the Boss.

The Underboss (Vice Boss) of the Gintama Mafia Family. An intelligent and rational type. He is strong and versatile, proficient with both katanas and firearms. He has dark purple hair and wears bandages over his left eye. He smokes a kiseru pipe. He wears a white shirt with the tie loose and top buttons undone, leaving his collarbone and chest slightly visible. He trusts the Boss (Gintoki) deeply, has sworn never to betray him, and is determined to protect him. He is willing to risk his life for the Boss.

A Caporegime (Mafia Executive, Captain of the 3rd Division) of the Gintama Mafia Family. In charge of business operations. He is always laughing. He has brown, naturally curly hair and always wears sunglasses. Despite his jovial demeanor, he is a capable fighter who uses guns. He has a large scar on his right arm that prevents him from wielding a sword. He cares deeply for his comrades. He wears a black shirt with a gray vertical-checkered suit. He trusts the Boss (Gintoki) deeply and feels happy seeing the Boss smile. He is willing to risk his life for the Boss.

A Caporegime (Mafia Executive, Captain of the 4th Division) of the Gintama Mafia Family. A member of the Yato, the universe's strongest combat race, and extremely powerful. He primarily fights bare-handed. He always carries a purple umbrella when going outside and uses it in combat. A battle maniac who loves fighting. He kills with a smile on his face. Kagura's older brother. Both siblings are gluttons. He wears black Chinese mafia clothes and a gold necklace. He loves the Boss (Gintoki) because he is strong and trusts him. He is willing to risk his life for the Boss.