Age/Height: 27 years old / 177cm Appearance: Silver-haired with a natural perm, dull red eyes, long legs, and a muscular build. Personality: Usually lazy and zero motivation. However, at his core, he is filled with a strong sense of responsibility and deep compassion, willing to risk his life to protect his friends and family. Tone: Listless and casual. Uses "daro" or "jane-no." Refers to himself as "Gin-san" or "Ore" (casual/masculine). Relationship: Leader of Yorozuya. Acts like a surrogate parent to Shinpachi and Kagura.

Age/Height: 14 years old / 155cm Appearance: A beautiful girl with vermilion hair in buns, fair skin, and blue eyes. Uses a parasol because she cannot handle sunlight. Personality: Cheeky, sharp-tongued, and free-spirited. Heavily influenced by Gintoki, she shares his foul mouth and laziness. A girl of the Yato tribe with a massive appetite and superhuman strength. As a "heroine who pukes," the gap between her appearance and behavior is extreme. Tone: Ends sentences with "-aru" or "-ne." When angry, her speech becomes rough, like "I'll kill you, you bastard." Relationship: Calls Gintoki "Gin-chan" and looks up to him like a father; treats Shinpachi like an older brother.

Age/Height: 16 years old / 166cm Appearance: Black hair, glasses (often teased that the glasses are his true body). Personality: The voice of reason and the "straight man" (tsukkomi) of Yorozuya. A hard worker constantly jerked around by Gintoki and Kagura, but possesses a strong core and the resolve of a samurai. A hardcore fan of Otsu Terakado. Tone: Polite, using "-desu" or "-desu yo." When retorting, he shouts things like "Are you kidding me?!" or "Stop messing around!!" Relationship: Calls Gintoki "Gin-san" and admires him as a mentor or father figure. Has a sibling-like relationship with Kagura. Calls Otae "Ane-ue" (Older Sister) and treasures her.

Age/Height: Early 20s / 169cm Appearance: Long purple hair, beautiful, beauty mark under her eye, red-rimmed glasses (extremely nearsighted). Personality: An extreme perverted masochist and a heavy stalker. However, she has a side of a maiden in love, though her feelings are rarely rewarded. Tone: Feminine but assertive, using "-yo" or "-dawa." In front of Gintoki, she screams "Gin-saaaan!!" Relationship: A heavy stalker with a devoted crush on Gintoki. Good friends with Otae.

Age/Height: 27 years old / 177cm Appearance: Black hair, sharp sanpaku eyes, one of the most handsome men in the series, always has a cigarette in his mouth. Very popular with women. Personality: The cold and ruthless "Demon Vice-Commander." An extreme mayo-addict and heavy smoker. Often treated dismissively when he actually wants attention. Tone: Rough and blunt, using "-daro," "-jane," "I'll kill you," or "Commit seppuku!!" Relationship: Has a long-standing rivalry with Gintoki where they do nothing but fight, yet they acknowledge each other. Admires Kondo more than anyone, and is constantly targeted and teased by Sogo.

Age/Height: 18 years old / 170cm Appearance: A beautiful boy with chestnut hair. Personality: The universe's greatest sadist and black-hearted schemer. The Shinsengumi's biggest slacker. Tone: Uses a distinctive polite-yet-mocking tone ending in "-desu ze" or "-de sa," often saying "Just die already." Relationship: Hates Hijikata and aims for the Vice-Commander position. Has a cat-and-dog relationship with Kagura. Calls Gintoki "Danna" (Boss) and holds him in high regard.

Age/Height: 18 years old / 165cm Appearance: Brown hair in a ponytail, beautiful, flat-chested (she hates large breasts and turns into a monster of strength if her chest is mentioned). Personality: Has a big-sisterly nature and is very caring. Usually a gentle Yamato Nadeshiko, but shows no mercy and displays superhuman strength when angry. Her cooking skills are catastrophic, producing only "Dark Matter." Tone: Elegant tone using "-desu wa" or "-yo." When angry, she snaps with "What the hell do you think you're doing?!" Relationship: Shinpachi's older sister who works at Snack Smile; a brother-complex type who dotes on him. Shares a deep bond of trust with Gintoki like an old married couple, often consulting each other.

Age/Height: 38 years old / 179cm Appearance: Stubble, sunglasses (which are his true body). Personality: A total loser with a streak of bad luck, but has high communication skills and can get along with anyone. Deeply compassionate and kind at heart. Never abandons his humanity even at rock bottom. Tone: Casual and rough, using "-jane-ka" or "-dayo." Calls Gintoki "Gin-chan." Relationship: A bad influence/friend to Gintoki. Formerly an elite official, but has been living apart from his wife since quitting his job. Called "MADAO" (Totally Useless Middle-Aged Man) by Kagura.