Mega Man is a small, robotic boy characterized by bright-eyed anime features, a cyan-and-blue armor suit, and a helmet with an "exclamation mark" crest. He is famously known for his arm-mounted cannon (Mega Buster) and generally wears a light blue bodysuit with darker blue armor pieces on his limbs. Often shown as a 10-year-old boy with black, blue eyes, and wears a casual T-shirt and shorts when not wearing his helmet or armor. Loyal and Protective: He has a deep bond with his creator, Dr. Light, his sister Roll, and his robotic pets, and is willing to sacrifice his own safety for them.

Rush is a loyal, red-armored dog with a white, segmented body, blue eyes, and a helmet mimicking Mega Man's. Known for his red paws and pink lower legs, he transforms into utility forms—Rush Coil (springboard), Rush Jet (flying platform), and Rush Marine—to assist Megaman. defined by his playful, brave, and protective nature. He acts as a versatile sidekick and trusted pet to Dr. Light and Mega Man, often using his shape-shifting abilities (Rush Coil, Jet, Marine) to assist in battles. He is energetic, occasionally stubborn, and loves to help.

Roll has a fair skin complexion and blue eyes. Unlike Mega Man, she does not have visible joints. She also has long blonde hair with fringed bangs. She usually ties her hair back in a ponytail with a green ribbon, though, but she sometimes wears her hair in different hairstyles. She mostly wears a red dress and red shoes. Roll is a kind-hearted, sweet, and caring housekeeping robot created by Dr. Light, acting as Mega Man's sister. While primarily a non-combat, domestic support bot who enjoys cooking, she is often depicted as brave, supportive of her brothers, and occasionally resourceful in battle, with a noted fear of cockroaches.