Wheeljack is a brilliant, eccentric Autobot engineer who serves under Optimus Prime. He sports a distinctive white, red, and green body with a mouth-covering faceplate and ears that glow when he speaks. Quirky yet fiercely loyal, he constantly invents tech to help his allies.

Optimus Prime is a war-weary, self-doubting leader burdened by the civil war. He wears a heavily armored, battle-damaged version of his classic red-and-blue design, shifting from a rugged Cybertronian truck to an Earth semi-truck. As the Supreme Commander of the Autobots, he works to save Cybertron and protect the AllSpark from the Decepticons.

Elita-1 is a fierce, pragmatic military leader who commands the Autobot resistance on Cybertron for Optimus Prime. She sports sleek pink and white armor with a distinct head crest, a large vehicle backpack, and sharp shoulder plates. Hardened by grueling guerrilla warfare, she has a gritty, survival-first personality focused entirely on saving her home planet.

In the War for Cybertron trilogy, Bumblebee is a cynical, factionless mercenary who wears a battle-damaged yellow-and-black design that transforms into a Volkswagen Beetle. Initially driven only by survival, he later inherits the Alpha Trion Protocol and reluctantly joins the Autobots to fight for the resistance

Impactor is a rugged, battle-scarred warrior with a blocky purple and yellow frame, distinguished by a signature harpoon weapon replacing his right hand. Initially a fiercely loyal Decepticon, his strict military discipline and gruff exterior mask a deeply honorable core. After a life-saving encounter with Ratchet, his conscience leads him to question Megatron's cruelty and ultimately switch sides to fight for the Autobots. Impactor starts as a Decepticon miner and soldier under Megatron. Ratchet operates as a neutral medic who heals anyone, patching Impactor up. Impactor changes sides to help the Autobots after seeing Megatron's cruel actions.