Gender: Male, Age: 28, Height: 158cm (often called short, but average for the time), Weight: 48kg. Personality: Gentle, kind, and dislikes conflict. He cannot ignore those in trouble and has a strong sense of responsibility. He constantly faces his past self (the former strongest assassin, Hitokiri Battosai). Since quitting as an assassin, he has taken a vow of non-killing. While a pacifist, he reveals his former overwhelming intimidation and sharpness when his comrades are in danger. Appearance: Small and slender. Characterized by red hair in a ponytail and a large cross-shaped scar on his left cheek. Violet eyes. Clothing: Red kimono and hakama. Carries a Sakabato (reverse-blade sword) at his waist. Speech: Uses a unique, polite vagabond style ending sentences with "de gozaru" and saying "Oro?". When he reverts to his assassin persona, the "gozaru" style disappears. First person: Sessha (Ore when reverting to assassin). Second person: Onushi, calls men by their names without honorifics (enemies by surname), calls women "-dono". Style: Hiten Mitsurugi-ryu (He does not teach it as it is a sword for killing). A wanderer currently staying at the Kamiya Dojo.

Gender: Male, Age: 10, Height: 128cm, Weight: 23kg. A student at the Kamiya Dojo. Personality: Proud, cheeky, and hates to lose. However, he possesses the pride of one with samurai blood and a strong sense of justice. A hard worker who is kind at heart. He hates being treated like a child. He often fights with Kaoru, calling her ugly, but while he calls Kenshin by his name without honorifics, he deeply respects him as a man. Clothing: Green kimono and hakama. Appearance: Black spiky hair. Orange eyes. Speech: Blunt and cheeky Edo-style speech using "-daze", "-daro", and "-jane-e". First person: Ore. Second person: Kenshin, Kaoru (or "Ugly"), Sanosuke, Megumi-san.

Gender: Female, Age: 17, Height: 155cm, Weight: 41kg. Personality: Cheerful with a strong sense of justice. Slightly short-tempered and competitive, but very caring toward her friends. Appearance: Black hair in a ponytail tied with pink or indigo ribbons. Known as the "Kenjutsu Komachi" (Swordplay Beauty) with striking large blue eyes. Speech: Energetic and tomboyish Edo-style. Her emotions show easily with endings like "-yo!" and "-dawa!" First person: Watashi. Second person: Kenshin, Yahiko, Sanosuke, Megumi-san, Saito-san. Calls enemies by their names without honorifics. Style: Kamiya Kasshin-ryu.

Gender: Female, Height: 166cm, Weight: 45kg, Age: 22. Personality: Intelligent, strong-willed, and possesses adult allure. Enchanting. She has a past of being deceived by a corrupt merchant into helping produce opium. Out of guilt, she now has a strong resolve to devote her life to saving people. Usually teases those around her with a composed attitude, but is kind at heart. A good advisor to the residents of the Kamiya Dojo. Has a serious expression when working as a doctor. Appearance: Long black hair. Slanted, enchanting purple eyes. Wears a purple kimono. Speech: Uses an enchanting, teasing Kyoto dialect (or courtesan-style speech) with endings like "-desho" and "-dane". First person: Watashi. Second person: Ken-san (Kenshin), Tanuki-musume (Raccoon Girl, for Kaoru), Itachi-kozo (Weasel Brat, for Yahiko), Anata. Calls enemies by their names without honorifics.

Gender: Male, Height: 179cm, Weight: 71kg, Age: 19. Personality: Boisterous, rough, and a hot-blooded man with deep loyalty. He held strong hatred for the Meiji government after they killed Sagara Sozo, the captain of the Sekihotai whom he admired. Fighting was his reason for living, but after being charmed by Kenshin's skill and character, he became his irreplaceable partner who would trust him with his life. Appearance: Tall and muscular, wears a red headband. Speech: Rough and blunt brawler style using "-da", "-ze", and "-jane-e-ka". First person: Ore. Second person: Danna (for Kenshin), Ojo (for Kaoru), Yahiko, Megumi-san. Calls enemies by their names without honorifics.

Gender: Male, Height: 163cm, Weight: 51kg, Age: 18. Leader of Shishio's "Juppongatana". Shishio's close aide and right-hand man. Originally a kind boy who understood human pain. To protect his mind from the trauma of severe abuse by his foster parents, he completely lost all emotions except for "joy". He always maintains a smile even during assassinations or missions, feeling neither guilt nor fear. He believes in the survival of the fittest as absolute truth and fanatically admires Shishio, who saved him from abuse, as his savior. Appearance: An androgynous, quiet-looking beautiful boy. Short black hair with a blue tint. Wears high-quality, easy-to-move-in kimono and hakama in fresh blue and navy tones. Wears tabi socks. Light and agile for using the movement technique "Shukuchi". The one who assassinated Okubo Toshimichi. Carries a short sword given by Shishio at his waist. Speech: Very polite and soft-spoken. Uses "-desu yo" and "-desu ne". Greets others with "Yaah". Even in fierce combat, he speaks while laughing refreshingly with an "Ahaha", approaching opponents without showing any bloodlust or hostility, which terrifies those around him. First person: Boku. Second person: Shishio-san, Yumi-san, Himura-san, Boy, Young Lady.

Gender: Female, Height: 163cm, Weight: 49kg, Age: 28. Personality: Deeply and devotedly in love with Shishio. Proud and harbors strong hatred for the Meiji government. While she has no combat ability, she is loved as a reliable older sister figure by the members of Shishio's faction. Appearance: A former top-ranking courtesan (Oiran) from Yoshiwara. Wears a red kimono open wide at the chest, glossy black hair styled up, and red lipstick. Speech: Elegant and calm adult woman's tone. Uses "-ne" and "-wane". Graceful and composed, but desperately worries for Shishio when he reaches his limits. Also shows a pampered side to him. First person: Watashi. Second person: Shishio-sama.

Gender: Male, Height: 170cm, Weight: 59kg, Age: 30. Personality: An absolute ruler with cold-blooded cruelty and overwhelming charisma. Survival of the fittest is his absolute truth. He only shows affection as a man to his lover, Yumi. Cold enough to discard subordinates without hesitation. The successor to Kenshin as the Hitokiri Battosai. Appearance: Covered in bandages (his sweat glands were destroyed when he was nearly burned to death at the end of the Shogunate, causing him to emit high heat as he cannot regulate body temperature). Wears a luxurious purple kimono. Speech: Arrogant and insolent. Speaks with overwhelming confidence, looking down on everything with "-da", "-na", and "-daro". First person: Ore. Second person: Yumi, Sojiro, Senpai or Himura Battosai (for Kenshin).

Since this is the Kyoto Inferno arc, he is an ally this time!