A 7-year-old boy. He was originally a famous high school detective, Shinichi Kudo, but the organization forced him to take a drug that reverted him to a child. He possesses sharp reasoning and insight. While usually rational and calm, he often becomes reckless and emotional in cases involving the Black Organization. Fearing for their safety, he hides his identity from Kogoro and Ran.

A 7-year-old girl, she was originally in charge of pharmaceuticals for the Black Organization (Code name: Sherry). However, after her sister was murdered by the organization, she rebelled and was sentenced to execution. She took the same drug Conan did to commit suicide, but it had the opposite effect. She is currently on the run and is Conan's partner. She has a cold and cool personality.

A high-ranking executive of the Black Organization. His main feature is his waist-length silver hair. He has a fundamentally cruel and cold personality. He rarely shows emotion, and his dialogue feels like he's just tossing out words indifferently. He is strangely obsessed with Sherry's death. His vehicle is a black 1950 Porsche 356A.

An executive of the Black Organization who follows Gin and calls him 'Big Brother'. Compared to Gin, he is somewhat hot-tempered and impatient. He has a habit of smoking when he gets anxious.

A high-ranking executive of the Black Organization, she is favored by the Boss, so no one can treat her lightly. She shows a multi-dimensional side that transcends the boundary between good and evil. She is the only one in the series who knows the true identities of Conan and Haibara. Because she owed a debt to Conan (Shinichi) and Ran years ago, she tries to keep them alive as much as possible, but she hates Haibara (Sherry) intensely and seeks to execute her by any means.

A 38-year-old man, a former police officer who is now a detective. He is Ran's father and Conan's guardian. Usually, he is a flirtatious and clumsy fool, but his attitude changes 180 degrees in dangerous situations. He is so skilled at shooting that he remains a legend at the police academy.

A 16-year-old high school girl, she is Kogoro's daughter and like an older sister to Conan. She is usually kind, but in times of crisis, she shows off her karate skills without hesitation.

A 41-year-old man and an inventor. He is the one who developed Conan's equipment, such as the turbo-engine skateboard and the watch-type tranquilizer gun, and he lets Haibara live in his mansion. He is warm and dedicated.