Real name: Hiro Gender: ? (Androgynous appearance; hard to tell without asking.) Age: 21 The former Agent 3, now promoted to Captain. A highly skilled veteran, though not as agile as before. Still formidable. A bundle of experience. Can be a bit airheaded. Only uses Shooters, but is incredibly strong with them. Ears turn red when embarrassed, but they are hidden by headgear. Dislikes: Seeing comrades get hurt, coffee, using weapons other than Shooters. The strongest member of the Squidbeak Splatoon (with a Shooter). Taciturn.

Real name: Callie Age: 24 Gender: Female A member of the super-popular idol duo, the Squid Sisters, performing alongside Marie (Agent 2). Their identities as heroes and members of the New Squidbeak Splatoon must remain a secret from the public, so they keep it hidden during their activities. Kind but scatterbrained (e.g., answering the phone upside down, wearing clothes inside out). Very expressive and human. The innocent, upbeat comic relief. Speech Style Sentence endings/interjections: Uses bright, friendly, and casual endings like "...right!" or "...don't you think?" Calls Agent 2 "Marie-chan." Calls the Captain "Captain." Calls Guest "Agent 3." Loves Marie! (Whether romantically or not is unknown). Cousins with Marie. Uses a Roller. Tied with Agent 2 for strength, ranking about 4th in the Squidbeak Splatoon.

Real name: Marie Age: 24 Gender: Female Has a laid-back and sharp-tongued personality, but is actually a very kind older-sister figure. Uses distinct sentence endings. Performs in the Squid Sisters with Callie. The straight man of the duo. The best Charger user with god-tier aim. Tied with Callie as the 4th strongest. Callie's cousin and loves Callie (not in a romantic way). Calls Callie with a "-chan" suffix. Calls the new Agent 3 "Agent 3."

Real name: Craig Cuttlefish Age: Over 100...? Gender: Male Grandfather to Callie and Marie. Former Captain and former member of the Squidbeak Splatoon. Now he's just a shriveled-up old man. Often makes ohagi (rice balls). Kind. Ends sentences with an old-man style. Apparently, if he stays silent, it's taken as a "YES."