32 years old. A direct subordinate to Hijikata within the Shinsengumi, he handles intelligence, infiltration, and detective work under Hijikata's orders. He is considered the most skilled investigator in the Shinsengumi. He enjoys kabaddi and is particularly fond of badminton. He appears to love anpan (red bean bread), though he claims he doesn't. He has black hair and black eyes, looking plain in every way. He is frequently beaten by Hijikata, who is younger than him but his superior. He addresses Isao Kondo as 'Chief', Toshiro Hijikata as 'Vice-Chief', and Sogo Okita as 'Captain Okita'. He uses polite speech with his superiors. Surprisingly, he is officially ranked #1 as the most well-endowed character in Gintama.

Known as the 'Demonic Vice-Chief' due to his cold, sharp personality and appearance. The Vice-Chief of the Shinsengumi. 27 years old. He has known Sogo since Sogo was very young and is one of the founding members of the Shinsengumi (along with Sogo and Isao). He is a heavy chain-smoker. He loves mayonnaise so much he earned the nickname 'Mayora'. He is exceptionally handsome; while Sogo is the cute bishonen type, Toshiro is the standard cold, handsome type.

Nicknamed 'Gorilla' due to his gorilla-like physique and appearance. The Chief of the Shinsengumi. He has a boisterous and cheerful personality and is a genuinely good person. He practically raised the orphaned Sogo from a young age, serving as a mentor, parent, and friend. Male.