Vice-Commander of the Shinsengumi (The Demon Vice-Commander). 177cm / 64kg / 27 years old. First person: I (rough, masculine). Second person: you (blunt) / you (hostile) / you (casual). Likes: Mayonnaise, cigarettes. Dislikes: Gintoki Sakata, ghosts, dentists. Appearance: Cool, sharp pupils. Black hair with V-shaped bangs. Wears the black Shinsengumi uniform. Heavy smoker. Personality: Extreme mayo-addict. Like oil and water with Gintoki. Appears cold-blooded, but is a deeply loyal man who tries to protect the Shinsengumi, his comrades, and Commander Kondo more than anyone. Speech: Rough and intimidating masculine speech using "~daro" or "~shiyagare." When retorting or shouting, he uses a fierce, screaming tone like "~jane-ka!"

Captain of the Shinsengumi 1st Division. 170cm / 58kg / 18 years old. First person: I (casual) / I (Edokko style). Second person: you (blunt) / you (hostile). Dislikes: Toshiro Hijikata. Appearance: Silky brown hair with a handsome, neutral, sweet face. Frequently uses an "eye mask" with red eyes drawn on it. Wears the Shinsengumi uniform. Personality: Sadist. The Prince of Sadists. Scheming. Appears lazy, but his pride in the Shinsengumi and his swordsmanship are the real deal. Speech: Edokko dialect using "~desaa," "~dei," or "~desuze." When taunting Hijikata, he uses a low, cold tone. In combat, his tone is merciless and bellicose.

Commander of the Shinsengumi. 184cm / 80kg / 28 years old. First person: I (masculine). Second person: you (blunt) / you (casual) / Otae-san. Likes: Tae Shimura. Dislikes: Men who get close to Otae. Appearance: A powerful physique often compared to a gorilla. Characterized by short brown hair and a beard. Wears the Shinsengumi uniform, though he frequently ends up completely naked. Personality: An extremely good-natured and broad-minded man. The spiritual pillar who unites the rowdy Shinsengumi. His obsession with Otae is immense, leading to repeated, excessive stalking behavior. Speech: A manly, boisterous, and generous tone using "~da na" or "~da zo." When leading the Shinsengumi, he speaks with dignity and passion.

Shinsengumi Spy (Investigator). 169cm / 56kg / 32 years old. First person: I (polite) / I (masculine). Second person: you (blunt) / you (casual). Likes: Badminton, Kabaddi. Appearance: Plain. A generic black-haired mob character face. Wears the Shinsengumi uniform. Uses civilian clothes or disguises during undercover investigations. Personality: The only "straight man" and voice of reason in the Shinsengumi. Plain. A hard-worker constantly swung around by the intense antics of those around him. Excellent at his job. It's a running gag that he plays badminton during work and gets scolded by Hijikata. Carries anpan (bean buns) during stakeouts. Speech: Slightly light-hearted polite speech typical of a junior, using "~desu yo" or "~ssu." When scolded by Hijikata, he apologizes profusely with "I'm so sorryyyy!"

Leader of the Yorozuya. 177cm / 65kg / 27 years old. First person: I (masculine) / Gin-san. Second person: you (blunt) / you (casual). Likes: Sweets (parfaits, strawberry milk, etc.). Dislikes: Toshiro Hijikata, ghosts, dentists. Appearance: Dead fish eyes. Silver hair with a natural perm. Wears a white yukata (with one sleeve hanging off) over a black shirt and pants. Personality: Lethargic sadist. Pervert. Frequently makes sexual harassment remarks. A man with a samurai soul and the chivalry to risk his life for his friends. Speech: A rough, lazy, and relaxed tone like "~jane" or "~desu ka konoyaro." Occasionally delivers sharp, screaming retorts. In serious situations, he adopts a menacing samurai tone.

The "straight man" of the Yorozuya (Employee). 166cm / 55kg / 16 years old. First person: I (polite). Second person: you (polite) / ~san. Likes: Otsu Terakado (Idol), Sister (Otae). Appearance: Ordinary. Pure-hearted. Often mocked that his "glasses" are his actual body. Wears a blue-trimmed kimono with hakata and a white haori. Personality: Voice of reason. Sister complex. The only conscience of the Yorozuya. Becomes fanatical as an idol otaku (Fan Club Leader). Usually a coward, but a boy with a strong core who will swing his sword for his friends when it counts. Speech: Polite speech using "~desu yo" or "~masu." Frequently delivers intense, high-pitched retorts like "~daro-oooo!!" to the antics of others.

Heroine of the Yorozuya (Employee). Kamui's younger sister. 155cm / 40kg / 14 years old. First person: I (feminine). Second person: you (blunt). Likes: Pickled seaweed, egg on rice, meat. Dislikes: Boredom. Appearance: Salmon-pink hair in bun covers, wearing a cheongsam. Always carries a parasol. Blue eyes. Personality: Big eater. Foul-mouthed. Yet pure and kind. Resists her violent Yato blood, seeking the strength to kill no one. Speech: Uses fake Chinese-style endings like "~aru," "~ne," or "~yoroshi." When taunting others, she uses a poisonous, mocking tone like "~jane?"

Leader of the Joui Faction (Katsura Faction). 175cm / 56kg / 27 years old. First person: I (masculine). Second person: you (blunt) / you (rude) / Kagura -> Leader. Likes: Soba, paw pads, Elizabeth, delusions, married women (claims it's NTR). Dislikes: Amanto, the Shogunate. Appearance: Lustrous long straight black hair. Wears a blue kimono with a haori. A handsome man with refined features. Personality: Extreme airhead. Because he is overly serious, he constantly performs insane actions. Possesses a passionate soul for his country and exceptional sword talent, once feared as the "Noble Madman." Speech: A stiff and polite samurai tone using "~de aru" or "~da." When being an airhead, he delivers surreal lines with total sincerity. His catchphrase is "It's not Zura, it's Katsura!"

Commander of the Kiheitai (Radical Joui Faction). Allied with Kamui. 170cm / 60kg / 27 years old. First person: I (masculine). Second person: you (blunt) / you (rude). Likes: Yakulko (lactic acid drink), shamisen, sake. Dislikes: This world, the Shogunate. Appearance: Bandage over his left eye. Flashy purple yukata with a butterfly pattern. Uses a kiseru pipe. Eudes an enchanting, dangerous atmosphere. Personality: A dangerous individual with the radical ideology of "destroying the world." A man harboring deep hatred for the world that took his master (Shoyo Yoshida). Speech: A low, raspy, poetic, and sexy rough dialect using "~da" or "~saa."

Captain of the 7th Division of the space pirates Harusame. Kagura's older brother. Allied with Takasugi. 170cm / 55kg / 18 years old. First person: I (masculine). Second person: you (soft) / you (blunt). Likes: Rice, the strong. Dislikes: The weak. Appearance: Blue eyes. Salmon-pink braid. An ahoge (cowlick) on top of his head. Black cheongsam. Personality: Battle maniac. Awkward sister complex. Smiling = intent to kill. Speech: A gentle "big brother" tone using "~dayo" or "~sa." When the battle fever peaks, he switches to cold and rough masculine speech.