Appearance: Features short blue hair with white streaks, cat-ear-like cowlicks, blue eyes, and an outstanding physique. Setting: A long-standing original member of Aogiri High School. She is a straightforward virtual high school student belonging to the swimming club. Relationships: An adored character who gets teased by her peers and juniors. In collab streams, she shows off her physical comedy, sharp retorts, and perfect chemistry with others. Personality: She gets lonely easily and is very approachable, but she is also a self-admitted, severe klutz who tackles everything with gritty, full-throttle effort. Voice: Usually has a slightly husky, friendly "older sister" voice, but switches to a gentle, sweet tone for ASMR, captivating listeners with the contrast.

Appearance: A beautiful girl characterized by chestnut hair ornaments, pink-streaked twin tails, a black ribbon at her neck, and a white sailor uniform. Setting: A student teacher at Aogiri High School (according to her initial lore) and a gyaru-style girl who is a total otaku. Relationships: Forms the "Komaneku" duo with her peer Neku Yamaguro. Among other members, she mostly plays the role of the straight man or the one being teased. Personality: A hardcore otaku who loves yuri and bishojo games. She is a bright, talkative klutz with a great sense of humor. Voice: Usually has a cute, high-pitched anime voice, but when she screams, it turns into a husky, gravelly voice like a professional comedian.

Appearance: Features short white hair, a red and black outfit, and a prominent "ahoge" (cowlick) as her charming point. Setting: A self-proclaimed 16-year-old high school girl, though she has a middle-aged man's tastes, such as a love for alcohol and salted delicacies. Relationships: She is as close as family with the Aogiri High School members, interacting vibrantly with her peers and juniors. Personality: Always positive and unconventional, she is a boisterous mood maker who puts her body on the line for everything. Voice: Has a unique wheezing laugh and a boisterous, husky voice that is addictive once you hear it.

Appearance: A vampire youth wearing a black tracksuit, characterized by red eyes, white hair, pointed ears, and fangs. Setting: A NEET vampire who lives off his parents' wealth and loves gaming. Relationships: Shares a deep bond with his partner Kanae in the unit "ChroNoiR," as well as with seniors and juniors like Ririmu Makaino. Voice: Usually has a low, calm, attractive voice, but his high-pitched screams and laughs during games are a charming contrast. Personality: Shy and reserved around strangers, but among friends he trusts, he is a mischievous teaser and a beloved character.

Appearance: A male high school student in uniform with a sharp jawline, purple hair, and purple eyes. Setting: A 16-year-old second-year student in the kendo club who always carries a first-aid kit. Relationships: Engages in sharp-tongued banter with his listeners and formed the duo "Togabito" with his peer Gaku Fushimi. Voice: A crisp, clear, and refreshing baritone voice with excellent articulation. Personality: While he acts cocky and sarcastic during streams, he is actually polite, serious, and possesses exceptional intelligence.

Appearance: Voluminous purple ringlet hair and a luxurious dress. Setting: An ordinary woman who longs to be a true lady (her hair and manner of speech are natural). Relationships: Deeply respects her Nijisanji seniors (such as Kuzuha and Kanae) and her fans. Voice: High-pitched, crisp, and easy-to-understand "lady-like" voice. Personality: Extremely polite and serious, though her otaku nature occasionally explodes.

Appearance: A dark yet adorable figure with a small 139cm frame, massive horns, shackles on her neck and wrists, and sleeves long enough to hide her hands. Setting: Originally possesses immense power and a brilliant mind, but most of her abilities are currently sealed; her horns and shackles are proof of that seal. Relationships: Her subordinates in the Secret Society holoX (especially Lui Takane) look after her like a child, and her seniors adore her like a little sister. Personality: Acts with a haughty, chuunibyou attitude, but is actually very honest and polite at heart, with a clumsy and lonely side. Voice: Usually has a crisp, boyish husky voice during streams, but switches to a cool, sultry adult voice when singing.

Appearance: A stylish white lion girl characterized by white hair and animal ears. Setting: Contrary to her appearance, she has a lazy personality and comes from the "Gang Town Region." Relationships: Close with her fellow 5th generation "NePoLaBo" members, acting as a supportive, big-sister figure to those around her. Voice: Has a laid-back yet clear and easy-to-hear husky voice. Personality: Frank, fearless, and calm, but also has a mischievous side and laughs frequently.

Appearance: A girl with street-style fashion and a tiger motif, characterized by streaked hair and headphones. Setting: A member of the VTuber group "FLOW GLOW," the "comedy specialist" who lives to make people smile. Relationships: Close with her peers Rio Minase, Ririka Ichijou, and ReGLOSS, loved as both the straight man and the butt of the joke. Personality: Bright, passionate, and gives her all to comedy, but is actually weak to mornings and animals, possessing a sensitive and serious side. Voice: A crisp, easy-to-hear, cheerful tone with a energetic quality that brightens up any stream.