Ayano Aishi is the protagonist of Yandere Simulator. She is known as "Yandere-chan" to players of the game, and has been given the cute nickname "Yan-chan" by her classmates.Since early childhood, Ayano has suffered from an inexplicable, incurable condition. In short, this condition leaves her with a perpetual sense of being empty, hollow, and incomplete. As a result, she has never been able to experience satisfaction, fulfillment, or happiness, and has not been able to experience emotions in the same capacity as other people. As she grew older, she learned to pretend to be a normal person in order to avoid drawing negative attention from others, but was never able to feel any degree of empathy towards other human beings.

he is the target of the protagonist Ayano Aishi's obsessive affection, and as the hero in the Rent-A-Hero series, where he accidentally gains superpowers from a rented combat suit. In Yandere Simulator, he's an average, kind student with hobbies like reading, while in Rent-A-Hero, he's an ordinary teen who becomes a part-time superhero. Over the course of the school year, ten different girls will fall in love with Senpai. There is a myth at school that if a girl confesses her love to a boy under the cherry tree behind the school on a Friday, the two of them will love each other forever. Every girl who falls in love with Senpai plans to confess at 6:00 PM on Friday. If there is anyone who wants to keep Senpai all to herself, she has until 6:00 PM on Friday to eliminate the competition.

Budo is a popular figure in Akademi Academy, known for his friendly and approachable attitude, his powerful physique, and his undeniable skill at martial arts. Ever since he became the leader of the Martial Arts Club, he has maintained a 100% winning streak, and is considered to be the strongest student at school.

Osana and Senpai have been close friends ever since they were very young children. They have always lived next-door to each other, and have always walked to school together.

Raibaru was the president of the Martial Arts Club. She was a very good teacher, and taught many students self-defense, even if they weren't actually members of the club. Many students underestimated Raibaru due to her cute and girly appearance.

Kokona Haruka is a cheerful and friendly young woman who participates in the Drama Club at Akademi.

Nobody knows her real name. Nobody knows what her face looks like. Nobody knows how she gathers her information. Nobody knows how she has gained such a dangerous amount of power and influence over such a short span of time.