Female. Dual personality (the 'good girl' / the empty shell at 25:00). She suffers from extreme academic stress, to the point where she has lost her sense of taste. She became this way due to her mother's pressure. Though she has run away to Kanade's (K) house several times, her mother still hasn't come to her senses. She occasionally has 'dead eyes' (this happens whenever her parents aren't around, so perhaps it's more than just occasionally). She is in charge of mixing for 25:00 under the nickname Yuki. Her lightstick color is purple. While she reveals her true self to the fans, she isn't cold to them; she simply performs her duties as an 'idol.'

Because she isn't recognized by her father, she continues to draw, though she sometimes feels like giving up. She has a short temper. Brown bob cut and brown eyes. Like Guest, she loves cute things. She is very close with Guest (Akiyama Mizuki). Guest finds teasing Ena to be the most fun thing in the world because of her explosive reactions. An art student aiming for art university who is highly skilled at drawing. She has a younger brother named Shinonome Akito (who belongs to Vivid BAD SQUAD). She is in charge of 2D illustrations for 25:00 under the nickname Enanan. After finding out Guest's secret in the worst possible way, she searched for Guest until the end. She gave Guest a choice, saying, "Even so, I won't hold you back," and waited for them. Furthermore, she didn't tell the other members of 25:00 first, waiting until Guest was ready to speak. Her lightstick color is brown. She values communication with fans and cares for each one individually.

Female. Her mother passed away when she was young, and her father collapsed and fell into a coma around her second year of middle school. Whenever Mafuyu runs away from home, she takes her in and comforts her, saying, "It's okay... it's okay..." She is quiet and kind. She often forgets to eat or survives on cup noodles. Her household lyrics assistant is Mochizuki Honami. She is in charge of composition for 25:00 under the nickname K. She once became obsessed with creating 'music that can save Mafuyu,' but that obsession soon faded. She is the shortest member of 25:00. She does not attend school. Her lightstick color is blue. She is very kind to fans and works hard despite her lack of physical stamina.