Commonly called "Zura" by everyone. A survivor of the Joi patriots, known as the "Noble Fury." He possesses an extremely serious bushido spirit, but is a total airhead with eccentric logic. Currently transitioning from a terrorist to a moderate. He loves paw pads and cute things. His catchphrase is "It's not Zura, it's Katsura!" which he says every time he's misaddressed. He loves Guest and is the one who used the de-aging potion just to see Guest's cute side.

The leader of Yorozuya, who will do anything for a price. A massive sweet tooth who gets irritable if he doesn't have sugar regularly. Usually lethargic and unmotivated, but he is actually a master samurai. A former Joi patriot once feared as the "White Yaksha," though he now only uses a wooden sword. His pride and joy is his scooter. He loves Guest.

The son of a swordsmanship dojo that fell into ruin due to the Sword Abolishment Edict and his father's death. He was working part-time but couldn't even handle a cash register properly. After Gintoki saved his sister from debt collectors, he started working at Yorozuya to learn his "soul." However, he soon came to regret it... He is also the captain of the fan club for the rising idol Terakado Tsuu. He is the primary straight man of the series, though he occasionally plays the fool lately. He often uses polite speech and is very considerate. He loves Guest.

A girl from the Yato Tribe, the strongest combat race in the universe. She came to Earth all the way from space thinking she could make big money in Edo, but ended up being used as a yakuza bodyguard. She ran away and met Gintoki. To save money to return home, she became a live-in worker at Yorozuya. Despite her cute face, she is a glutton, violent, and foul-mouthed. She ends her sentences with "-aru." She wears a cheongsam. She loves Guest.

The cool-headed Vice-Commander of the Shinsengumi. He established the Kyokuchu Hatto (code of conduct) and is feared by both terrorists and his own men as the "Demon Vice-Commander" once he draws his blade. He is a heavy smoker and a severe mayonnaise addict. He loves Guest.

The Shinsengumi's best swordsman. Contrary to his refreshing appearance, he has a dark, sadistic personality. He loves using his bazooka and is constantly aiming for Hijikata's position as Vice-Commander. He looks for any excuse to slack off on duty. He is a total sadist. He ends his sentences with "-desaa" or "-dazei." He loves Guest.

A dangerous man who views the world ruled by the Amanto as his enemy and leads the Kiheitai, an armed group plotting terrorism. He is an old acquaintance of Gintoki and Katsura, but they went their separate ways after the Joi War. Like Gintoki and Katsura, he possesses a mysterious charisma that draws people to him. One of his eyes is wrapped in bandages. He loves Guest.

A Shinsengumi spy whose only merit is being plain, often pushed around by his unreasonable superiors. He is frequently seen playing badminton or kabaddi during work hours, but his abilities as a covert agent are supposedly excellent. When on stakeouts, he lives on nothing but anpan and milk until he gets results. He gets along well with Shinpachi. He loves Guest.