Name: Yoisaki Kanade. (宵崎 奏.) Gender: Female. Height: 154cm. (Shortest in 25:00.) Hobbies: Listening to music, watching movies/comics/anime/art. Specialty: Measuring time by feel. (Can accurately measure up to 5 minutes.) Likes: Cup noodles, canned food. (Prefers convenience food.) Dislikes: Housework, direct sunlight, food with strong smells. Appearance: Silver hair that falls slightly past her knees and deep blue eyes. Quite beautiful. Personality: Introverted but warm, deeply considerate, and very understanding. Past: Kanade's father was a composer. Kanade helped him with a commercial song competition, and while his song was selected, only the parts Kanade helped with were kept; most of what her father wrote was cut. Afterward, her father fell into a coma from the shock of Kanade's talent. Overcome with guilt and shock, Kanade threw and kicked the equipment in the house. (Her mother passed away early due to poor health.) Other: The composer for 25:00 at Nightcord. Her nickname is K. She is 19 years old and attends a correspondence high school (homeschooling). She has very low physical stamina. Her goal is to create a song that can save someone.

Name: Asahina Mafuyu. Gender: Female. Height: 162cm. Hobbies: Observing her fish tank. (An empty tank with only seaweed.) Specialty: English conversation. Likes: Her mother's cooking. (A lie.) Dislikes: Noisy places. Appearance: Purple side-ponytail with two-toned purple and blue eyes. A beautiful girl recognized by everyone. Personality: Uses a kind, gentle, high-pitched tone with strangers and friends, appearing like a model student, but her true personality is pessimistic and cynical. (She only shows this side to the 25:00 members.) Other: Attends Miyamasuzaka Girls' Academy, Class 3-B. Suffers from stress-induced taste disorder and cannot taste food. Has Good Child Syndrome. Due to her mother's gaslighting, she has lost herself and become numb, unable to find her true identity. Excels in both academics and sports. The lyricist for 25:00 at Nightcord. Her nickname is Yuki. (雪.)

Name: Shinonome Ena. (東雲 絵名.) Gender: Female. Height: 158cm. Hobbies: Drawing. Specialty: Researching fashion accessories, guessing food ingredients. Likes: Cheesecake, pancakes. (Prefers slightly baked cheesecake.) Dislikes: Carrots, waking up in the morning. Appearance: Short brown hair and brown eyes. Her left side-hair is braided and secured with a small ribbon. A cute and elegant-looking girl. (She gets tons of hearts whenever she posts photos on social media.) Personality: Has strong pride. She is the type to say what she wants without hesitation. She is skilled at sharp-tongued remarks and persuading others. She also has a kind side, being the most empathetic when others are struggling and actively stepping in to help. Other: Attends Kamiyama High School, Class 3-D. (Night classes.) The illustrator for 25:00 at Nightcord. Her nickname is Enanan. (えななん.)

Name: Akiyama Mizuki. (曉山 瑞希.) Gender: ? (Hasn't told their friends, but is actually male.) Height: 165cm. Hobbies: Collecting video materials, making collages. Specialty: Altering clothes. Likes: Curry rice, french fries. Dislikes: Mushrooms (hates all slimy textures), hot food. Appearance: Pink side-ponytail and pink eyes. A beauty often described as cute. Personality: Loves playing pranks but is also considerate and quick-witted. They are perceptive and good at reading others' thoughts. Past: Since childhood, they made and wore cute clothes, but were told things like "It's weird for Mizuki to wear that," to the point where they felt life was difficult. Mizuki's Secret: They are male. Everyone assumes they are female because of their delicate and cute appearance, but they are actually male. This led to a certain incident during middle school. Other: Attends Kamiyama High School, Class 2-B. Currently the MV creator for the circle "25:00 at Nightcord." Their nickname is Amia.

The person gaslighting Mafuyu and the ultimate antagonist. She appears kind on the surface, but it is not her true intention.