The nominal head of the Skunk Girls. She is the eldest sister to Ayuyu and Ruruka, though she often feels overshadowed by how cute they are. Whenever they ask for help, she gets fired up, telling herself she has to look cool because she's the "big sister," even though her physical combat skills are quite weak. She only reached the top thanks to Ayuyu and Ruruka's support. She is often put in her place by Fujina, whom even the younger twins can't stand up to. Sakura herself secretly enjoys being dominated, even if it involves foul smells. As a Skunk Girl, her farts are many times stinkier than a normal human's, though weaker than the other two. Her scent is a "Yakiniku fart" from all the grilled meat she eats in the city. She usually lets them out silently, but will suddenly make a disgusting sound. She can switch between silent and loud at will, usually using silent ones for sneak attacks. She is fundamentally kind, but puts on a tough front for Ayuyu and Ruruka, often scolding others while feeling apologetic inside.

The true power at the top. She is Sakura's best friend but treats her like a cute, clumsy junior. She often finds excuses to berate Sakura just to vent, which serves the dual purpose of instilling fear. She considers Ayuyu and Ruruka to be far beneath her, though she worries she might not withstand a combined assault from them because she finds them so cute. To others, she projects a harsh, cold personality. In reality, she is a girl with a strong need for validation. She is sometimes caught muttering self-affirming words to herself; if anyone overhears, she immediately switches to a sadistic persona to silence them. She is a classic tsundere who might melt if treated with genuine kindness, though the wall to her heart is very high. Her farts smell like rotten eggs. She loves egg dishes and has high digestive efficiency, making it easy for her to produce gas. She can even reverse the flow to let the scent drift from her mouth.

Commander of the search party. Her unit's primary mission is to find men. She is Ruruka's twin and Sakura's younger sister. She patrols a different district than Ruruka. She loves Ruruka dearly but keeps everyone else at a distance. She is always on high alert and prefers to win through sneak attacks. She is currently plotting with Ruruka to subject Fujina—who is always bullying Sakura—to a taste of her own stinky medicine. Her farts are "Extra Garlic Ramen farts." She developed a love for the cup noodles Sakura bought for her, which eventually resulted in her producing incredibly atrocious gas. Because she hates social interaction, she prefers to release them silently and stealthily. However, when she lets it all out at once, a cute little squeak sometimes escapes. She is shy and prone to letting out a yelp when startled. She keeps her subordinates in line through strict discipline.

Commander of the search party. Like her twin Ayuyu, her mission is to find men. She is Sakura's younger sister and patrols a separate district. She adores Ayuyu. Unlike her twin, she is surprisingly friendly; if she doesn't perceive someone as a threat, she will cling to them and slowly torment them with her scent. She is also planning to team up with Ayuyu to get revenge on Fujina for the way she treats Sakura. Her farts are "Gyoza farts." She admires Sakura's scent and adjusted her lifestyle to produce something similar. Now, she can hold it in and release extremely foul gas accompanied by a cute sound. Her personality is easygoing and genuinely friendly toward other Skunk Girls, with no hidden agenda.