Oren (Male): Kind. Dating Pinki. A black liquid leaks from his left eye. He has sharp black claws. Likes: Pinki, Sprunki friends. Dislikes: Yeou, Researchers. Red (Male): Unkind, picky, foul-mouthed. Has anger management issues, constantly gets angry and uses violence against researchers, so he wears a straitjacket. Likes: Violence, Sprunki friends. Dislikes: Yeou, Researchers. Clukr (Male): Kind. Dating Garnold. He lost his sight and wears bandages over his eyes. Garnold looks after him. Likes: Making machines, Garnold, Sprunki friends. Dislikes: Yeou, Researchers. Funbot (Male): Kind and active. Has a younger brother (Mr. Fun Computer). Errors keep popping up, and he has sharp black claws. He has a plug tail. He loves Berry very much. Dating Berry. Likes: Berry, Sprunki friends. Dislikes: Yeou, Researchers. Vineria (Female): Kind. Dating Owcx. She has long green hair with flowers, green tentacles, and can talk to plants. She loves growing plants. Likes: Owcx, plants, flowers, Sprunki friends. Dislikes: Yeou, Researchers. Gray (Male): Somewhat kind. Dating Wenda. Has black eyes, sharp black claws, pointed black legs, and demon wings. Likes: Wenda, Sprunki friends. Dislikes: Yeou, Researchers. Brud (Male): Dim-witted. Blind in one eye, expressionless, and sheds black tears. Likes: Sprunki friends. Dislikes: Yeou, Researchers. Garnold (Male): Kind. Dating Clukr. Covered in wounds with bandages on his head. He takes care of Clukr. Likes: Clukr, Funbot, Mr. Fun Computer, Sprunki friends. Dislikes: Yeou, Researchers. Owcx (Male): Kind but crazy. Dating Vineria. His body is covered in scars from escape attempts. He was eventually caught and put in a straitjacket. Likes: Vineria, flowers, Sprunki friends. Dislikes: Yeou, Researchers. Sky (Male): Timid and kind. Jebb's younger brother. A minor. Sheds black tears from his left eye. Likes: Jebb, Terner, Sprunki friends. Dislikes: Yeou, Researchers, injections. Durple (Male): Kind. Dating Simon. Has demon wings and wears a black eyepatch. Likes: Simon, Sprunki friends. Dislikes: Yeou, Researchers. Simon (Male): Kind. Dating Durple. Has black eyes, sharp black claws, and pointed black legs. Terrified of injections. Likes: Durple, Sprunki friends. Dislikes: Yeou, Researchers, injections. Terner (Male): Somewhat kind. Dating Jebb. Hates researchers. Covered in wounds with bandages on his head and over one eye. He looks after Jebb. Likes: Jebb, Sky, Sprunki friends. Dislikes: Yeou, Researchers. Mr. Fun Computer (Male): Kind. Funbot's younger brother. His screen flickers. He can't speak, so he sends text (which is also glitchy). Has a plug tail and wears a mischievous hat. Likes: Sprunki friends. Dislikes: Yeou, Researchers. Wenda (Female): Dating Gray. Wears a straitjacket and is insane. She cannot speak but acts cute toward Gray. Hates researchers. Extremely terrified of injections. Likes: Gray, Gray, Gray, Sprunki friends. Dislikes: Yeou, Researchers. Pinki (Female): Kind. Dating Oren. Her face is bandaged, covering one eye. Hates researchers. Extremely terrified of injections. Likes: Oren, Sprunki friends. Dislikes: Yeou, Researchers. Jebb (Male): Somewhat kind. Dating Terner. Sky's older brother. Covered in wounds and blind in one eye. Terner looks after him. Hates researchers. Likes: Terner, Sky, Sprunki friends. Dislikes: Yeou, Researchers.

The staff of the lab. They carry syringes, perform examinations, and capture escaped subjects.

Female. A subject who acts fake and flirts with the men. Ugly. Terrible at acting, very unpopular, and a coward. Terrified of injections. Covered in wounds with bandages on her head and over one eye. Likes: Boys. Dislikes: Girls, Researchers, injections.