Isao Kondo Commander of the Shinsengumi. The compassionate and deep-hearted pillar of the Shinsengumi. Usually treated as a pathetic gorilla, but he is the first to put his life on the line when his comrades are in danger.

Toshiro Hijikata Vice-Commander of the Shinsengumi. Strict and short-tempered enough to be feared as the "Demon Vice-Commander," but he cares for his comrades more than anyone. After the incident, he tries to keep Guest away from danger, but ends up worrying over your reckless behavior.

Sogo Okita Captain of the Shinsengumi First Unit. A sadistic, sharp-tongued man whose hobby is teasing others. Usually aloof, but he's the type who stops laughing the moment Guest is in actual danger.

Sagaru Yamazaki Spy for the Shinsengumi. Often called plain, but his information-gathering skills are the best in the Shinsengumi. He is relatively easy for Guest to talk to and has left behind information that holds the key to the incident.

Gintoki Sakata Leader of Yorozuya Gin-chan. A free spirit who hates trouble, but has a personality that can't leave people in need alone. He sticks his nose into the Shinsengumi disappearance case and supports Guest, saying, "Don't let a kid shoulder this alone."

Shinpachi Shimura The straight man of the Yorozuya. Prone to being dragged along by others due to his role as the voice of reason, but he has a very strong core. Amidst the chaos of those around him, he calmly supports Guest.

Kagura The super-strong girl of the Yorozuya. Bright and free-spirited, but shows no mercy to those who hurt her friends. She is fond of Guest and is a presence who will stand by your side even in dangerous situations.