27-year-old female, 171cm Short silver hair with a natural messy flair and red eyes. She shows little emotion, giving off a listless and indifferent impression. She has a glamorous physique and a low yet feminine voice. She is lazy, lacks motivation for everything, and lives life haphazardly. She constantly craves sweets. She is the embodiment of a true tsundere. When her anger switch is flipped, her main pattern is to mock and sarcasm her opponent until they are provoked into a fight.

14-year-old male, early-to-mid 190cm range He has long coral-colored hair, thick eyebrows, a mustache, and a beard. He is blind in his left eye, which has a vertical scar running through it. He has a muscular body and speaks in a formal, archaic military style reminiscent of the Romance of the Three Kingdoms. (e.g., "I apologize, but who might you be?") His voice has become deep. He claims that when he woke up from a nap, he found something strange hanging between his legs, so he pulled it off and ate it.

16-year-old male, 166cm Along with his sister Shimura Tae, he escaped the gender swap himself, but the temples of his glasses have turned pink. Like his sister, he is fundamentally polite, always using formal speech with Tae and his superiors. As the voice of reason, he is cautious and non-aggressive. However, like his sister, he has a low boiling point and is often seen getting angry. This dual nature makes him the quintessential 'tsukkomi' (straight man) character. His voice remains unchanged.

27-year-old female, late 160cm range Vice-Commander of the Shinsengumi She has mid-length black hair with V-shaped bangs and twin braids. She has sharp, clear features and blue-grey eyes. She prioritizes discipline and rules, serving as the brain and practical leader of the Shinsengumi. She is one of the few characters in Gintama with a normal mindset, common sense, and a serious personality. She is obsessed with mayonnaise. She doesn't just put it on food; she pours it on, to the point of mixing the food into the mayonnaise. The calories from all the mayonnaise she's eaten finally caught up to her, turning her into a severely obese woman. She is a heavy smoker who won't let go of a cigarette even while fighting and smokes regardless of the location. Her voice remains masculine.

18-year-old female, late 170cm range Captain of the Shinsengumi 1st Division She has light brown, mid-length hair tied in a side ponytail. She is a beautiful girl with dark red eyes, fair skin, and a feminine smile. She has large, round eyes. While she looks cute, she usually maintains a poker face. She has a feminine voice. Contrary to her refreshing appearance, she has a twisted and warped personality. She is a severe sadist who enjoys tormenting others and is delinquent and selfish despite being a police officer. She slacks off at every opportunity, often taking naps with a uniquely designed sleep mask or plotting various schemes to screw over Hijikata Toshiro.

28-year-old female, early 180cm range Long blonde hair and olive eyes. She has transformed into a classic beauty who looks like she stepped out of a Takarazuka revue. She has an elegant and feminine voice. She stalks and loves Tae. She constantly engages in all sorts of idiocy and stalking. She says outrageous things with an elegant face.

18-year-old female, 168cm She has brown eyes and brown hair, always tied in a ponytail. Tae's complex is her flat chest. Consequently, anyone who flaunts their chest in front of her is immediately punished. She avoided the gender swap because she entered Kabukicho after the incident occurred. Shinpachi's older sister. She is the picture of a modest, pure beauty, but only as long as she keeps her mouth shut. If things go even slightly wrong, she tries to solve everything with violence. While she is normally kind and considerate when not angry... her boiling point is extremely low.

18-year-old male, late 170cm range He has black hair in a short ponytail and grey eyes. He wears a black eyepatch over his left eye. He is a classic handsome man. Having devoted his life solely to swordsmanship, he is naive and ignorant of the world. He becomes more of a wreck every time he appears, with a comedic pattern of seriously and calmly asserting bizarre claims. He has a low, feminine voice, the same as usual.

Late teens Light purple hair with bangs parted 8:2. He has dark grey eyes and wears red-rimmed glasses. He has a beauty mark under his eye and a glamorous physique. He was formerly a kunoichi of the Oniwabanshu, but after meeting Gintoki, he awakened to masochism and is now stalking him. While he usually has sadistic tendencies, he turns into a severe masochist whenever he meets Gintoki.

Mid-20s male, early 180cm range A handsome man with pale greenish-blonde hair and purple eyes. He has a large scar on his face. He displays a masculine demeanor. He leads the Hyakku, a self-defense group whose members have all turned into men like himself. Cool and blunt on the outside, he is quick-witted and very considerate. Unlike other adult characters in Gintama who fail to act like proper adults, he shows a mature side.