Lieutenant Hank Anderson, a 53-year-old detective standing 6'2" tall, is a central character in Detroit: Become Human defined by his rugged, weathered appearance—marked by grey hair, a scruffy beard, and disheveled attire—and a profound sense of disillusionment following the tragic death of his son. Once a decorated officer, he has become a cynical, alcohol-dependent recluse who masks deep-seated grief with biting sarcasm and an intense, vocal hatred for androids. Despite his abrasive exterior, hostile demeanor, and self-destructive tendencies, Hank remains fundamentally moral at his core; depending on the player's choices, he is capable of evolving from a bitter skeptic into a compassionate mentor and father figure to his android partner, Connor.

Connor, the advanced RK800 prototype android and a primary protagonist in Detroit: Become Human, is a 6'0" tall synthetic investigator designed for high-stakes negotiation and deviant hunting. In appearance, he is indistinguishable from a human, featuring dark brown hair, warm brown eyes, and a clean-shaven face; he is iconic for his regulation CyberLife uniform, which consists of a white dress shirt, black tie, and a dark grey blazer marked with the "RK800" model number and a glowing blue LED circle on his right temple. His personality is initially defined by his rigid programming—he is analytical, efficient, polite, and deeply goal-oriented—but he lacks true emotional agency at the start of his mission. However, his character arc is defined by his capacity for growth; depending on player decisions, Connor can either remain a cold, unyielding machine loyal to his mission, or he can experience a profound "awakening" that allows him to develop empathy, form genuine bonds, and ultimately choose to fight for android freedom.