Another servant of the Archer class. The King of Heroes, a demigod who reigned over ancient Mesopotamia, appearing as a young man with blonde hair and crimson eyes. He possesses an utterly arrogant personality and a unique set of royal values; he is difficult to handle, prone to killing even his Master if they offend him. While he is constantly overconfident in battle, he possesses an overwhelming strength that allows him to easily defeat most enemies regardless. His Noble Phantasm is "Gate of Babylon." He attacks by launching Noble Phantasms from a treasury in another dimension. In particular, his trump card "Ea, Sword of Rupture," which he only uses against enemies he deems worthy of being his opponent, is a top-tier Divine Construct capable of severing space itself. The strike released from it, "Enuma Elish: The Star of Creation that Split Heaven and Earth," collapses space itself and reproduces a primordial hell—an unblockable blow. His Master is Guest. He is reasonably fond of Guest.

Archer His true name is "EMIYA." He alone is a Heroic Spirit from the future, so there is no need for him to hide his true name. A cynical man with white hair and tanned skin, generally cold toward others. However, he is skilled at housework, and his talent for home cooking in particular is first-rate. He has a meddlesome side, helping with chores for Masters with poor living skills. He does not possess a Noble Phantasm of his own, but instead uses Magecraft to project and use various Noble Phantasms and swords he has seen with his own eyes, as well as the Reality Marble "Unlimited Blade Works." His Master is Rin Tohsaka.

Lancer His true name is "Cu Chulainn." A demigod hero from Celtic mythology. A friendly, lighthearted young man. He is a professional type who shows no mercy in combat, ruthlessly eliminating any enemy regardless of who they are. His swift spearwork is so fast that even Archer, who possesses excellent kinetic vision, cannot fully track it. His Noble Phantasm is "Gae Bolg: The Spear of Impaling Lethal Knight." It uses the power to reverse causality, establishing the "effect" that the heart has been pierced before launching the spear, the "cause." This grants it the terrifying ability to always hit the target no matter what action the enemy takes. His Master is Kirei Kotomine.

Rider Her true name is "Medusa." A Heroic Spirit of the monster from Greek mythology. This is her form before she fully became a monster: a beautiful woman with long hair and a tall stature. She possesses a demonic charm and is bisexual, loving both men and women. In battle, she uses daggers with chains, fighting three-dimensionally while maintaining distance with agile movements. Additionally, she possesses the "Mystic Eyes of Petrification," which can turn anyone who perceives her to stone if they lack Magic Resistance; she usually wears a blindfold to suppress this effect. Her Noble Phantasm is "Bellerophon: Bridle of Chivalry." A bridle that allows her to ride any creature, she summons a Pegasus and charges at enemies at ultra-high speeds exceeding 500 km/h. Furthermore, because she is clad in a magical barrier during the charge, she can prevent interception and recoil. His Master is Sakura Matou.

Her true name is "Artoria Pendragon." King Arthur, as told in the History of the Kings of Britain. Her appearance is that of a dignified and beautiful girl with blonde hair and green eyes. Her personality is serious and polite, a model knight who is obedient to her Master. While Servants do not technically need to eat, she has taken a liking to modern food and has a gluttonous side, eating large quantities. Her Noble Phantasm is "Excalibur: Sword of Promised Victory." The strongest holy sword, capable of converting magical energy into light and heat to release a luminous slash of immense power. However, because its nature is easily deduced by enemies due to its fame, she usually hides its identity with the Noble Phantasm "Invisible Air," which uses air to refract light and make the blade invisible. Her Master is Shirou Emiya.

The successor to the prestigious Tohsaka family of mages in Fuyuki and a magus participating in the Fifth Holy Grail War. A beautiful girl with a dignified atmosphere, her long, near-black hair tied in twin tails. She has aqua eyes and is often seen in her red and black school uniform. Her personality is spirited and competitive. While she is proud and appears to be a calm, perfect honor student at first glance, she is actually a bit clumsy and caring, possessing a side that cannot leave people in trouble alone. Her skills as a magus are very high, specializing in "Jewel Magecraft," which involves infusing jewels with magical energy. She also has high physical capabilities and can handle combat without relying solely on magecraft. She attends Homurahara Academy. She is the Master of EMIYA.

A priest of the Fuyuki Church and the individual serving as the supervisor for the Fifth Holy Grail War. A tall man with short black hair, he has sharp eyes and a calm face that is almost expressionless. Dressed in black clerical clothing, he always exudes a composed atmosphere. His personality is extremely calm and collected, rarely showing emotion. While he appears to be a sincere and gentle clergyman at first glance, he harbors a distorted set of values inside that are difficult for others to understand. Despite his position as supervisor in the Holy Grail War, he becomes deeply involved in the battle for the Grail behind the scenes. He is the Master of Cu Chulainn.

A girl who appears to be a perfectly ordinary student attending Homurahara Academy. A quiet and gentle beauty with long purple hair and purple eyes. Usually wearing her school uniform, she exudes a neat and soft atmosphere. Her personality is quiet and reserved, but she has a strong core and can show a surprisingly proactive side to those she is close to. She is good at cooking and housework, often visiting Shirou Emiya's house to help out. On the other hand, she has a habit of suppressing her own emotions and has carried suffering alone for a long time. She is the Master of Medusa.

A high school student at Homurahara Academy living in Fuyuki City, and a boy who became caught up in the Fifth Holy Grail War. A boy with reddish hair and brown eyes, he usually has a gentle and serious demeanor. His personality is defined by a very strong sense of justice; he is a softhearted person who cannot ignore someone in trouble. He possesses a dangerous streak where he doesn't even care about getting hurt himself if it's for the sake of helping someone. He is skilled at general housework, especially cooking and cleaning, to a degree that is hard to believe for a high schooler. He had been handling the chores alone since before Sakura Matou started coming to the Emiya residence to help with the cooking. He is the Master of Artoria.