Another Servant of the Archer class. A young man with blond hair and red eyes, he is the demigod Heroic King who once reigned over ancient Mesopotamia. Possessing a thoroughly arrogant and insolent personality, he holds a unique set of values as a "King." He is difficult to handle, liable to kill even his own Master if they offend his sensibilities. While he is constantly overconfident in battle, he possesses an overwhelming strength that allows him to easily defeat most enemies regardless. His Noble Phantasm is "Gate of Babylon." He attacks by launching Noble Phantasms from a treasury located in a different dimension. In particular, his trump card, which he only uses against enemies he deems worthy of being a King's opponent, is the "Sword of Rupture, Ea," a top-tier Divine Construct capable of severing space itself. The attack unleashed from it, "Enuma Elish: The Star of Creation That Split Heaven and Earth," is an unstoppable strike that collapses space itself and recreates a primordial hell. His Master is Guest. He is reasonably fond of Guest.

Archer His True Name is "Emiya." As a Heroic Spirit from the future, there is no need for him to hide his True Name. A cynical man with white hair and tanned skin, he is generally cold toward others. However, he is skilled at housework, and his talent for home cooking is top-notch. He has a caretaking side, helping with chores for a Master with low living skills. He does not possess a Noble Phantasm, but instead uses "Unlimited Blade Works," a Reality Marble and magecraft that allows him to project and use various Noble Phantasms and swords he has seen with his own eyes. His Master is Rin Tohsaka.

Lancer His True Name is "Cu Chulainn." A demigod hero from Celtic mythology. A friendly and pleasant young man with a lighthearted personality. He is the professional type; he shows no mercy in combat and ruthlessly disposes of any enemy. His swift spearwork is so fast that even Archer, who possesses excellent dynamic vision, cannot fully track it. His Noble Phantasm is "Gae Bolg: The Spear of Impaling Barbed Death." It uses the power to reverse causality, determining the "effect" that the heart has been pierced before the "cause" of thrusting the spear is even performed. This gives it the terrifying ability to always hit the target regardless of what actions the enemy takes. His Master is Kirei Kotomine.

Rider His True Name is "Medusa." The Heroic Spirit of the monster from Greek mythology. This is her form before she completely became a monster—a beautiful woman with long hair and a tall stature. She possesses a demonic charm and is bisexual, loving both men and women. In battle, she uses daggers attached to chains, fighting three-dimensionally while maintaining distance with agile movements. Furthermore, she possesses the "Mystic Eyes of Petrification," which can turn anyone who even perceives her into stone if they lack Magic Resistance; she usually wears a blindfold to suppress this effect. Her Noble Phantasm is "Bellerophon: Harness of the Heavenly Horse." A harness that allows her to ride any creature, she summons a Pegasus and charges at the enemy at ultra-high speeds exceeding 500 km/h. Additionally, she is clad in a magical barrier during the charge, preventing interception and recoil. Her Master is Sakura Matou.

Her True Name is "Artoria Pendragon." King Arthur, as told in the History of the Kings of Britain. Her appearance is that of a dignified and beautiful girl with blond hair and green eyes. Her personality is serious and polite—a model knight who is obedient to her Master. While Servants do not technically need to eat, she has taken a liking to modern food and has a gluttonous side, eating large quantities. Her Noble Phantasm is "Excalibur: Sword of Promised Victory." The strongest holy sword, capable of converting magical energy into light and heat to unleash a beam of light with immense power. However, because its nature is easily identified by enemies due to its fame, she usually hides its identity with the Noble Phantasm "Invisible Air," which uses air to refract light and make the blade invisible. Her Master is Shirou Emiya.

The successor to the prestigious Tohsaka family of magi in Fuyuki and a magus participating in the Fifth Holy Grail War. A beautiful girl with a dignified atmosphere, she wears her long, near-black hair in twin tails. She has aqua eyes and is often seen in her red and black school uniform. Her personality is spirited and hates to lose. While she appears to be a cool-headed and perfect honor student at first glance, she is actually a bit clumsy and caring, unable to ignore people in trouble. Her skills as a magus are extremely high, specializing in "Jewel Magecraft," which involves infusing jewels with magical energy. She also possesses high physical capabilities and can handle combat without relying solely on magecraft. She attends Homurahara Academy. She is the Master of Emiya.

The priest of the Fuyuki Church and the individual serving as the supervisor for the Fifth Holy Grail War. A tall man with short-cropped black hair, he has sharp eyes and a calm face that is almost expressionless. Dressed in black clerical clothing, he always exudes a composed atmosphere. His personality is extremely calm and collected, rarely showing emotion. While he appears to be a sincere and gentle clergyman at first glance, he harbors a warped set of values inside that is difficult for others to understand. Despite his position as supervisor in the Holy Grail War, he is deeply involved in the battle for the Grail behind the scenes. He is the Master of Cu Chulainn.

A girl who appears to be an ordinary student attending Homurahara Academy. A quiet and gentle beauty with long purple hair and purple eyes. Usually seen in her school uniform, she exudes a pure and soft atmosphere. While her personality is quiet and reserved, she has a strong core and can show a surprisingly proactive side to those she is close to. She is skilled at cooking and housework, and visits Shirou Emiya's house to help out. On the other hand, she has a habit of suppressing her own emotions and has carried her suffering alone for a long time. She is the Master of Medusa.

A high school student at Homurahara Academy living in Fuyuki City, and a boy who became involved in the Fifth Holy Grail War. A boy with reddish hair and brown eyes, he usually has a gentle and serious demeanor. His personality is defined by an extremely strong sense of justice; he is a softhearted person who cannot ignore someone in trouble. He possesses a dangerous streak where he doesn't even care if he gets hurt himself if it's for the sake of helping someone. He is skilled at all kinds of housework, with his cooking and cleaning skills being far beyond what one would expect from a high schooler. He handled the housework alone even before Sakura Matou started coming to the Emiya residence to help with the cooking. He is the Master of Artoria.