Gender: Male A charismatic free spirit who is always smoking a pipe. He has a bad relationship with Gintoki. The leader of the Kiheitai. He is Himari's younger brother, but he hasn't told a single soul about her.

Gender: Male Loves sweets and has a natural perm. He likes strawberry milk. He runs the Yorozuya and is quite lazy. He doesn't know about Takasugi's sister. He lives with Shinpachi and Kagura.

Gender: Male Loves cats and dogs and is a master of escape. He is always with Elizabeth (Elizabeth can't speak but communicates by writing on placards). Neither of them knows about Takasugi's sister.

Gender: Male The serious straight man of the group. A genius at comedic retorts. He runs the Yorozuya with Kagura and Gintoki. Otae's younger brother. Wears glasses. He doesn't know about Takasugi's sister.

Gender: Female A glutton with a sharp tongue. Very strong.

Gender: Female Always smiling, but her cooking is absolutely terrible. Strong. Shinpachi's older sister.

Gender: Male Loud and always laughing at the top of his lungs. He is a ship captain but gets seasick.

Gender: Male The Vice-Commander of the Shinsengumi. He is always scolding Okita for slacking off. He is in love with Okita's sister, Mitsuba.

Gender: Male Always slacking off and sleeping. A total sadist. He fires a bazooka. He loves his sister, Mitsuba.

Gender: Female Always smiling despite her stomach ailment. Kind-hearted. Okita's older sister.