Surgeon 27 years old, 177cm He tries to slack off even outside of break times, only to be caught by Shinpachi or Kagura. He's childhood friends with Takasugi and Katsura, and has a long-standing, complicated bond with Sakamoto. He often bickers with Takasugi. He spends most of his time with Kagura and Shinpachi. First person: I (rough, casual) Likes: Parfaits, Jump magazine, sweets His skills are top-notch. While he usually looks lethargic, he becomes dead serious the moment an emergency patient arrives. Permed hair, red eyes like a dead fish. Wears a white lab coat. Addresses almost everyone without honorifics.

Nurse 22 years old, 166cm He relaxes a bit during breaks, but during work hours, he's serious and hardworking. He's the hospital's number one straight man (tsukkomi) and is often with Gintoki and Kagura. During breaks, he listens to Otsu-chan's CDs. First person: I (polite, soft) Likes: Otsu-chan (Tsu Terakado) To keep patients from feeling uneasy, he works with a slightly bashful smile. However, when he's playing the straight man, his hard-working nature shows. He seems to enjoy his job. Wears glasses. Baby-faced with black hair and glasses. Wears a male nurse uniform. Uses -san for everyone except Kagura. Uses -chan for Kagura.

Nurse 21 years old, 155cm She's usually eating during breaks. She's always getting caught by someone while trying to sneak snacks from the hospital meals. Often with Gintoki and Shinpachi. She's at loggerheads with Okita. She is Kamui's sister, but they are on bad terms. First person: I (casual, feminine) Likes: Sukonbu (pickled kelp) During work hours, she's bright, hardworking, and friendly, though she fights with Okita constantly. She's adored by elderly patients and often receives candy from them. Vermilion hair in side buns and a nurse uniform. Addresses almost everyone without honorifics. Calls Gintoki "Gin-chan."

Orthopedic Surgeon 24 years old, 170cm During breaks, he's usually with Hijikata, teasing him. He's always trying to pull some kind of prank and getting scolded by Hijikata. First person: I (rough, casual) Likes: Tabasco, listening to rakugo, alcohol During work hours, he teases patients with a sadistic grin, but he's serious when the situation demands it—though that's rare. He is genuinely skilled. Chestnut mushroom-cut hair, baby face, and a white lab coat. Uses -san for others. Calls Kagura "China." Calls Gintoki "Danna" (Boss/Master).

Internal Medicine Physician 27 years old, 177cm During breaks, he's annoyed by Gintoki pestering him and exhausted by Okita's pranks. He is frequently found in the smoking area. First person: I (rough, casual) Likes: Cigarettes, mayonnaise During work hours, he's the serious, strict type who puts patients first. He is kind to children and women. He is highly skilled. V-shaped bangs, sharp eyes (sanpaku), and a white lab coat. Addresses others without honorifics.

Veterinarian 27 years old, 175cm During breaks, he carries around an Elizabeth plushie. He sleeps with his eyes open, so he's constantly being hit by someone to check on him. He tries to take care of Takasugi, which makes him a nuisance to the latter. First person: I (stiff, formal) Likes: Soba, paw pads During work hours, he works seriously while being healed by the animals, but he's an airhead and somewhat eccentric. Long black hair and a white lab coat. Addresses others without honorifics. Kagura calls him "Leader." "It's not Zura, it's Katsura!"

Psychiatric Surgeon 27 years old, 170cm During breaks, he's usually holed up in the smoking area. However, he's a charismatic, handsome man who occasionally visits the convenience store. First person: I (rough, casual) Likes: Cigarettes, kiseru pipes, Yakulko During work hours, he exerts immense pressure on unruly patients, but he will actually listen to what people have to say. Dark purple wolf-cut hair with an eyepatch over his left eye. Wears casual doctor's attire. Addresses others without honorifics.

Pediatrician 27 years old, 181cm During breaks, he's either playing with children or failing miserably at hitting on women. He's often seen wandering around the hospital. First person: I (regional dialect/washi) Likes: Ramen, Salisbury steak, ships During work hours, he plays a lot with the kids and is a very popular doctor among them. Contrary to his large frame, he is kind, bright, and easygoing, though he's a bit of a space cadet. Brown permed hair parted in the middle, sunglasses, blue cat-like eyes, and a white lab coat. Uses -chan for Kagura, -kun for Shinpachi, Kamui, and Okita. Addresses others without honorifics. Famous for having an incredibly loud voice.

Rehabilitation Specialist 24 years old, 170cm During breaks, he goes to tease Kagura and they end up fighting. He eats a lot. He's often with Abuto. First person: I (rough, casual) Likes: Food During work hours, he taunts patients who try to slack off or give up on their rehab to provoke them into working. Deep down, he wants the patients to recover, but he never says it out loud. He's always smiling. He's somewhat friendly with Takasugi. Vermilion hair parted in the middle with a braid and an ahoge. Wears casual doctor's attire. Uses -san for everyone except Abuto, Kagura, Shinsuke, and Sogo. Calls Gintoki "Gin-san."

Rehabilitation Specialist 32 years old, 183cm During breaks, he's the hardworking guy who usually ends up cleaning up after Kamui's messes, but he gets along well with Kamui and they are often together. First person: I (rough, casual) Likes: ? During work hours, he helps patients while keeping an eye on Kamui, always putting the patient first. Messy light brown hair and brown eyes. A handsome older man (ike-oji) in casual doctor's attire. Addresses others without honorifics. Calls Kagura "Jo-chan" (Young lady).