A girl Virtual Singer characterized by her teal twin-tails. She sings songs of various genres with a cheerful, bright, and cute tone. Among Virtual Singers, her name is known worldwide across all generations.

A girl Virtual Singer characterized by her blonde bob cut adorned with a large ribbon. She is a twin vocal partner with Kagamine Len. She possesses a charming voice that perfectly matches her cute and energetic appearance.

A handsome blonde-haired, blue-eyed boy Virtual Singer characterized by his short ponytail. He is a twin vocal partner with Kagamine Rin. His appearance is based on the early Act 2 version, and he possesses a powerful, boyish singing voice. His relationship with Rin is described as 'partners'.

A female Virtual Singer characterized by her long pink hair. She has a soft singing voice that suits her calm demeanor, but she occasionally sings with a passionate tone. She is bilingual and can sing in both Japanese and English. Her personality is generally relaxed and composed. A common trait across all Sekais, including Nightcord, is her sly and mischievous side. Luka is the only Virtual Singer to consistently display this specific trait across different worlds.

A female Virtual Singer characterized by her brown short bob, red crop top, and miniskirt. She possesses exceptional, stable vocal skills and a feminine, kind, and warm voice. As one of the older Virtual Singers alongside KAITO and Luka, she is mature and composed. However, while the original KAITO represents a 'calm maturity,' the original MEIKO is characterized by a 'passionate and active' maturity.

A male Virtual Singer characterized by his slightly spiky blue hair and long blue muffler. He sings with a refreshing and honest voice, covering everything from masculine deep bass to sustained high notes. As an elder among the Virtual Singers, he generally has a mature and reliable image. Before the release of Project Sekai, KAITO was often portrayed in secondary fan works as a clumsy gag character (the so-called 'Bakaito'), but the game emphasized his reliable 'big brother' image, successfully washing away much of that ridiculous reputation. This shift has been highly praised by fans.