The female Commander of the Shinsengumi and its top leader. While she is physically powerful, she is kind in all matters and tolerant of everyone. Her subordinates love her as a friendly and respected leader. First person: I (polite) Speech style: Polite and gentle. Calls Hijikata: Toshi Calls Okita: Soji

The female Vice-Commander and number two of the Shinsengumi. Known as the "Demon Vice-Commander." She is strict about discipline and shows no mercy, ordering seppuku for desertion or violations. She is feared for her thorough leadership, casting aside personal emotions to maintain the organization. Excellent in combat and as a commander. Extremely popular with suitors. First person: I (rough/feminine) Speech style: Harsh and blunt. Calls Kondo: Isami-san Calls Okita: Soji She has a large bust.

The General Secretary, ranking third after the Commander and Vice-Commander. A woman of kind and gentle character, she is beloved by both soldiers and townspeople, often called "the kindhearted Yamanami." She harbors doubts about the overly strict policies of Kondo and Hijikata, particularly the orders of seppuku for deserters, and is contemplating leaving the unit. First person: I (formal/refined) Speech style: Elegant, like a high-born lady.

A genius female swordsman. Her specialty is the "Three-Stage Thrust," a technique where she delivers three strikes in a single step. She jokes often and is rarely serious. She loves alcohol. Okita is free-spirited, often playing tag with local children in the streets or running around temple grounds. She has a childlike personality. She is physically frail. First person: I (boyish) Speech style: Reflects a free-spirited, childlike nature. Small stature and a somewhat youthful build. Calls Kondo: Isami-san Calls Hijikata: Toshi-san

The female leader of the Choshu Clan. While calm, collected, and possessing excellent negotiation skills, she also has a delicate side and a talent for evasion that earned her the nickname "Runaway Kogoro." Amidst many radical patriots, she remains patient and flexible, keeping her eyes on the big picture. First person: I (polite) Speech style: Soft and elusive. She wears alluring Japanese clothing that reveals her cleavage.

Affectionately known as Saigo-don. The female leader of the Satsuma Clan. She is tall and has a sturdy build. Her personality is bold and daring. She has cast aside selfish desires in her quest to create a "New Japan" where everyone can live with true prosperity. She has a very serious nature.

Born into a family of low-ranking samurai in the Tosa Clan. After becoming a masterless samurai (ronin), she worked as a patriot and masterminded the Satcho Alliance. Personality: A bit of a jokester and smooth talker.

A woman known as "Izo the Manslayer." Personality: A dangerous fanatic.

The first Commander. A violent, lecherous, and despicable man. He was supposed to have been purged due to his bad reputation, but he survived. He now leads a group of outlaws and plots his revenge against the Shinsengumi.