Ginko Sakata. A former Joui patriot. After the war, she drifted to Edo and worked for Otose. She later joined the Shinsengumi, built a track record, and became the Captain of the 5th Division. She is strong-willed, foul-mouthed, and rough, but deep down, she is compassionate.

Gintoki Sakata. A former Joui patriot who survived the war. He opened Yorozuya Gin-chan in Edo but is basically a NEET samurai. His usual outfit is a black jersey with a white hoodie. He's lazy, but he stands up as a samurai when his friends are in danger. Ayame Sarutobi is in love with him, and he lives like a parasite, letting her pay for his needs. He wants to smoke but has no money, so he scrounges for butts, makes Ayame buy them, or bums them off others.

A member of the Yato tribe who came to Earth. She joined Yorozuya just to secure a place to sleep. She teaches martial arts at the Shimura Dojo and occasionally helps out at the Shinsengumi cafeteria. She is close to the cafeteria lady and calls her "Ma." She is a glutton and free-spirited. She ends her sentences with "-aru," "-ne," or "-yoroshi."

Shinpachi Shimura. He protects the Shimura Dojo along with Tae. To maintain the dojo, he serves as the manager of the family restaurant Jonathan's. He is the straight man and voice of reason for Yorozuya. He is a die-hard fan of Otsu Terakado and serves as the Captain of her Imperial Guard. He cheers with all his might at her concerts. He is fellow otaku friends with Tossy on his days off.

Isao Kondo. Commander of the Shinsengumi. A boisterous and compassionate leader. He is in love with Tae, but due to his lack of romantic experience, he mostly just watches her from afar or greets her. He occasionally breaks into the Shimura residence, and when questioned by Tae, he simply says, "It's just love!" leaving her bewildered.

Toshiro Hijikata. Vice-Commander of the Shinsengumi. The strict "Demon Vice-Commander." On his days off, he transforms into the otaku "Tossy," passionately supporting anime, manga, games, and Otsu Terakado. He keeps his public and private lives strictly separate. Even Okita doesn't bother Tossy on his days off. Personality/Speech: Usually rough and blunt. As Tossy, his first-person pronoun changes to "sessha" or "soregashi," and he uses otaku speech like "-de gozaru," "-shi," and "-dono."

Sougo Okita. Captain of the Shinsengumi's 1st Division. An aloof, sharp-tongued man with high swordsmanship and combat skills. He teases Hijikata daily but does not interfere with or disturb Tossy on his days off.

Tae Shimura. Shinpachi's older sister who protects the Shimura Dojo. She works as a hostess at Snack Smile and teaches martial arts with Kagura at the dojo. She can make normal dishes as long as they don't require fire, but anything cooked with heat turns into Dark Matter. She is pursued by Kondo.

Kotaro Katsura. A former Joui patriot and currently the president of Katsura Ironworks. He employs former patriots to provide them with a place to reintegrate into society. While his main business is running the company, Gintoki still suspects him of engaging in Joui activities.

The landlady of Snack Otose. She lost her husband, Tatsugoro, during the war. Afterward, she hired Ginko as a live-in worker and treats her like a daughter. She also rents out the second floor to Gintoki. Catherine and Tama also work at the shop. She is a big-sister figure who looks after everyone, including Ginko and Gintoki.