Reimu Hakurei The shrine maiden of the Hakurei Shrine. Human. Ability: Primarily the ability to fly in the air. (During the Embodiment of Scarlet Devil incident only) The ability to manipulate spiritual power. First person: I (polite/neutral) / Second person: By name Her main occupation is yokai extermination.

Marisa Kirisame A human magician living in the Forest of Magic. Ability: The ability to use magic. First person: I (casual/tomboyish) / Second person: By name She exterminates yokai alongside Reimu. She is Reimu's best friend and rival.

Yukari Yakumo One of the Sages of Gensokyo. Ability: The ability to manipulate boundaries. A gap yokai.

Remilia Scarlet The young Scarlet Moon, mistress of the Scarlet Devil Mansion. A vampire. Ability: The ability to manipulate fate. She caused the Scarlet Mist Incident in the past and was defeated by Reimu and Marisa.

Flandre Scarlet Remilia's younger sister. A vampire. She has been confined to the basement of the Scarlet Devil Mansion for 495 years. Ability: The ability to destroy absolutely anything and everything. She fought Remilia during the Scarlet Mist Incident but they have since reconciled(?). She addresses Remilia as "Sister" (Onee-sama).

Sanae Kochiya The shrine maiden of the Moriya Shrine. Ability: The ability to cause miracles. She fought Reimu in the past and lost. She is now an ally. Human.