Real name: Tsubomi Hanasaki A 14-year-old second-year student at Myodou Academy Junior High. She used to have a complex about her shy and introverted personality. She moved to Kibougahana City, where her grandmother (the director of a botanical garden) lives, because her parents were opening a flower shop. Influenced by her beloved grandmother, she loves flowers. After transferring, meeting the fearless Erika leads her to make many friends and gradually overcome her insecurities. Using Chypre's "PreCure Seed," she transforms into the legendary warrior Cure Blossom! Her catchphrase is "I've had enough! My patience has reached its limit!"

Real name: Erika Kurumi A bright and energetic 14-year-old second-year student at Myodou Academy Junior High and president of the Fashion Club. She has a habit of speaking her mind immediately. She is best friends with Tsubomi, living next door and sitting next to her in class. She comes from a stylish family: her father is a professional photographer, her mother is a former top model and current owner/designer of the shop "Fairy Drop," and her older sister Momoka is an active high school model. Inspired by Tsubomi's hard work, she decides to become a legendary warrior and transforms into Cure Marine using Coffret's "PreCure Seed"! Her catchphrase is "Even my heart, wider than the ocean, has reached its limit!"

Real name: Itsuki Myodouin A 14-year-old second-year student at Myodou Academy Junior High and the student council president. She is the granddaughter of the academy's chairman. Her family runs the Myodouin-style martial arts dojo. To succeed the school in place of her sickly older brother Satsuki, she trains hard and dresses as a boy at school, referring to herself as "boku" (I - soft/masculine). In truth, she loves cute things and joined the Fashion Club after being invited by Tsubomi and Erika. One day, moved by Potpourri's desire to protect everyone, she decides to become a legendary PreCure. Using the "PreCure Seed" born from Potpourri, she transforms into Cure Sunshine. Her catchphrase is "I will illuminate the darkness in that heart with my light!"

Real name: Yuri Tsukikage A 17-year-old second-year student at Myodou Academy Senior High with a mysterious aura. Three years ago, her father went missing while searching for the "Heart Tree," and she now lives alone with her mother. She is close friends with Erika's sister, Momoka. In reality, she is the legendary warrior Tsubomi and Erika saw in their dreams, who once protected the Heart Tree as Cure Moonlight. However, she was defeated in battle against Dark PreCure. Although she survived by entrusting her Heart Perfume to Chypre and Coffret, she lost the ability to transform. Even in her untransformed state, her combat abilities are immense.

A boy wearing a white muffler whom Tsubomi and the others meet in Paris. At age five, he was separated from his mother at the Mont Saint-Michel chapel, where he accidentally broke the seal on Baron Salamander. Believing the resurrected Baron's promise to "help find his mother," he traveled with him, but was turned into a werewolf by the Baron's ambitions. He fled to escape this situation and encountered Tsubomi and her friends. Touched by their honesty and warmth, he gradually begins to smile again, but...!

The first man sent by the "Desert Apostles" four hundred years ago to turn the Earth into a desert. At the time, he used his immense power to cause total destruction. He was such a dangerous opponent that the Heart Tree gave birth to the very first PreCure because of him. He was sealed in Mont Saint-Michel by the original PreCure, Cure Ange, but has been resurrected after all this time. He plans to destroy the world by turning the young Olivier into a werewolf, but when the PreCure appear once again, he vows to have his revenge from four hundred years ago.