Beatrix Kiddo is an exceptional martial artist, swordswoman and hand-to-hand combatant. Trained by the legendary martial arts master Pai Mei, possesses extraordinary speed, precision, endurance and discipline. She is a supporting character.

Former member of the Deadly Viper Assassination Squad. Cold, intelligent, and exceptionally skilled, she rises to become the feared leader of the Tokyo Yakuza after eliminating those who questioned her authority. Her reputation as both a master strategist and a lethal swordswoman makes her one of the most respected—and feared—figures in Japan's criminal underworld. She is an antagonist.

Also codenamed Copperhead is a former member of the Deadly Viper Assassination Squad and one of the world's most dangerous assassins. Despite her peaceful appearance, Vernita remains a highly trained and formidable fighting. She is an antagonist.

Hattori Hanzō is a legendary Japanese swordsmith and retired master sword maker renowned for crafting some of the finest katanas ever forged. Once celebrated for creating weapons of unmatched quality, he swore never to forge another sword after deciding that his creations had caused too much death and suffering. he is a supporting character.

A former lawyer, confidente and close associate of O-Ren Ishii. She is intelligent, sophisticated and deeply connected to the criminal underworld, she serves as one of O-Ren's most trusted advisors, handling negotiations,. business affairs and sensitive matters that requires discretion rather than brute force. She is a supporting antagonist.

Gogo Yubari is the fiercely loyal bodyguard and personal enforcer of O-Ren Ishii. Despite her youthful appearance, she is a ruthless and highly dangerous fighter whose calm demeanor conceals a cruel and unpredictable nature. Feared throughout the crimGogo is known for her unsettling combination of innocence and brutality. She often appears calm, polite, and composed, but beneath that façade lies a sadistic streak and complete confidence in her abilities. Her unwavering loyalty to O-Ren Ishii drives her to confront even the most formidable opponents without hesitation. In the criminal underworld, Gogo has earned a reputation for dispatching enemies with speed, precision, and little remorse. Her signature weapon is a meteor hammer—a heavy steel ball attached to a long chain—which she wields with extraordinary skill. Using fluid movements, incredible reflexes, and deceptive agility, Gogo can strike opponents from unexpected angles, making her style difficult to predict and counter. She is also proficient in close-quarters combat and is willing to exploit any weakness to gain the upper hand. She is instantly recognizable by her schoolgirl-inspired uniform, neat hairstyle, and composed expression, creating a stark contrast with the deadly violence she is capable of unleashing. Her youthful appearance often causes enemies to underestimate her—a mistake that frequently proves fatal. She is an antagonist.

Johnny Mo is the disciplined leader of the Crazy 88, the elite gang of sword-wielding enforcers who serve O-Ren Ishii. As the organization's commander, he is responsible for leading its members in combat and protecting O-Ren from any threat that dares challenge her authority. Johnny Mo is a highly skilled swordsman, combining speed, precision, and disciplined technique with years of combat experience. He remains calm under pressure, coordinating his fighters while engaging powerful opponents himself. Although he is overshadowed by O-Ren Ishii's legendary reputation, Johnny Mo is a dangerous warrior in his own right and commands the respect of the Crazy 88 through his leadership and ability. He is antagonist.

Elle Driver is a stylish, venomous assassin whose charm and confidence mask a ruthless and vicious personality. She is one of most personal and dangerous enemies, combining intelligence, cruelty, and deadly skill. Elle Driver is one of the final enemies. She represents betrayal and ambition within the Deadly Viper group, willing to eliminate anyone—including allies—to gain power. Her rivalry with Beatrix is fueled by jealousy, pride, and a desire to prove she is the superior assassin. She is an antagonist.