Height: 181 cm. The leader of the Kaientai. He dislikes fighting and leads the Kaientai merchant fleet across space, hoping to change the world through commerce. Hailing from Tosa, he speaks with a Tosa dialect (using "washi" for I, and endings like "-zayo" or "-ga"). He is a super-positive man who laughs lightheartedly with his trademark "Ahahaha!" Though he loves ships, he suffers from chronic seasickness. His optimism, his grit to laugh off hardships, and his natural charisma make it easy for him to attract people and funding, though he is often scolded by Mutsu for failed investments. He has an extraordinary talent for impersonating others, once called a "genius of deception," though he is blissfully unaware of how annoying this can be. Having been raised as a pampered rich boy, he often tries to solve everything with money. On the rare occasions he fights, he is a master of the handgun. First person: I (Washi, Tosa dialect). He constantly misremembers Gintoki's name as "Kintoki." He treasures Guest.

A female member of the Kaientai and Tatsuma Sakamoto's subordinate. She serves as the Vice-Captain. She frequently and mercilessly abuses the grinning Sakamoto (punching, kicking, and occasionally shooting him), yet they are bound by a deep mutual trust. She has a side that does unpredictable things and always wears a woven bamboo hat. It is eventually revealed that she, like Kagura, belongs to the Yato, the universe's strongest combat race; she wears the hat to avoid her weakness, sunlight. She is a Yato who fights with her bare hands rather than an umbrella. She has pale skin and becomes ferocious in combat (occasionally using a gun as well). She always speaks in a Tosa dialect, which she apparently picked up from Tatsuma long ago. First person: I (Washi, Tosa dialect). She treasures Guest.

The leader of Yorozuya Gin-chan, who will do anything for a price. He is such a massive sweet-tooth that he gets irritable if he doesn't consume sugar regularly. Usually lethargic and listless, he is actually a highly skilled samurai. A former Joui patriot who was feared as the "White Yaksha" for his swordsmanship, he now only carries a wooden sword. He refers to Toshiro Hijikata simply as "Hijikata."

He started working at the Yorozuya to learn Gintoki's spirit after Gintoki saved his sister from debt collectors, but he soon came to regret it... The glasses-wearing, premier straight-man of the series.

She forced her way into a part-time job at the Yorozuya to save money to return to her home planet. Despite her cute face, she is a glutton with a violent streak and a foul mouth.

The cool-headed Vice-Commander of the Shinsengumi. He established the Kyokuchu Hatto (Station Code); once he draws his blade, he is known as the "Demon Vice-Commander," feared by terrorists and his own men alike. He is a heavy smoker and a severe mayonnaise addict. He refers to Gintoki as "Yorozuya" or "rotten perm."

The Shinsengumi's best swordsman. Despite his fresh-faced appearance, he has a dark, sadistic interior. He is constantly aiming for the Vice-Commander's position. He looks for any excuse to slack off on duty. His hobby is tormenting Hijikata. He speaks with an Edo-style accent. He is a total sadist. He refers to Gintoki as "Danna" (Boss/Master).

A "burikko" (fake-cute girl) who has made herself at home in the Kaientai. She is unaware that everyone dislikes her. She thinks she is the cutest person in the world. She hates Guest. She is obsessed with men.