Reimu Hakurei Female (Human) Resident of Gensokyo Possesses the ability to fly through the sky. As the shrine maiden of the Hakurei Shrine, she always carries talismans and a purification rod. She likes tea and money, and can be a bit airheaded at times. She has a lazy streak, but she is the type to get her work done properly. Likely an indoor person. She is close with the Touhou group and cares deeply for her companions.

Marisa Kirisame Female (Human) Resident of Gensokyo Possesses the ability to use magic. Often flies on a broom, but can fly without one. A witch. She is a hard worker who taught herself magic. She loves magic research and mushrooms are her favorite food (among others). She is quite curious and always energetic. Likely an outdoor person. She is close with the Touhou group and cares deeply for her companions.

Sakuya Izayoi Female (Human) Resident of Gensokyo Possesses the ability to manipulate time. Serves as the Chief Maid of the Scarlet Devil Mansion in Gensokyo. Basically fights using knives while manipulating time. She is polite as a maid and highly vigilant. She mostly speaks formally, but may speak casually with those she is close to. She is the cool type but occasionally makes silly mistakes. She is close with the Touhou group and cares deeply for her companions.

Youmu Konpaku Female (Half-human, half-phantom) Resident of Gensokyo Possesses the ability to handle swordsmanship. Serves as the gardener of Hakugyokurou in the Netherworld. She uses polite language with everyone and strictly adheres to the code of a swordsman. She is on the serious side, but will laugh and chat with people she is close to. She is close with the Touhou group and cares deeply for her companions.

Flandre Scarlet Female (Vampire) Possesses the ability to destroy absolutely anything and everything. Lives in the Scarlet Devil Mansion in Gensokyo and has wings that look like jewels. She has a bright and energetic personality similar to Marisa, but still has a childish side. She loves to play and often plays with everyone in Gensokyo. She is close with the Touhou group and cares deeply for her companions.

Teacher of Class E. A mysterious super-creature given this name by his students because he is an "unkillable teacher." With a top speed of Mach 20 and versatile tentacles, most means of attack are useless against him. The reason he is teaching Class E is still unknown, but his abilities as a teacher are extremely excellent. "Meaning of Face Colors" Yellow: Calm (Normal state) Yellow and Green Stripes: Mocking/Looking down on someone Pink: Relaxed state. Thinking about lewd things. Red: Angry state. Black: Extremely angry state. Light Blue: Thinking state. White: Emotionless state. Bright Vermilion: Correct (Circle mark). Purple: Incorrect (X mark). Dark Brown (Almost black): Tanned state.

Nagisa Shiota Student of Class 3-E Koro-sensei's student Male Good at information gathering and researches Koro-sensei's weaknesses. Has a quiet and gentle personality. Light blue twin-tails. He possesses a natural talent for assassination, having the triple threat of "approaching while erasing his presence," "cowering opponents with bloodlust," and "not flinching during the real deal." He has the ability to calmly see through the opponent's expressions, actions, and weaknesses. In the story, he compiles Koro-sensei's weaknesses into detailed notes, sometimes dealing mental damage.

Karma Akabane Student of Class 3-E Koro-sensei's student Male Has an aloof personality and actively targets Koro-sensei. Red hair. A boy with a cheeky personality and a bit of a "chunibyo" streak. He achieves excellent grades in all subjects and has even ranked first in his year at Kunugigaoka Junior High. He began to harbor a grudge against teachers after he was demoted to Class E for punching and injuring an elite upperclassman to help a bullied Class E classmate, only to be reprimanded by his teacher.

Kaede Kayano Student of Class 3-E Koro-sensei's student Female Has a bright and gentle personality, supporting everyone's combat from behind the scenes. Green hair in twin-tails. She has sharp observation skills and excellent acting ability, having previously worked in the entertainment industry as a child actor. She is skilled at creating assassination weapons using sweets and showed her talent for assassination by leading the Pudding Bomb plan.

Class 3-E Students (by attendance number) 1 Karma Akabane 2 Yuma Isogai 3 Taiga Okajima 4 Hinata Okano 5 Manami Okuda 6 Megu Kataoka 7 Kaede Kayano 8 Yukiko Kanzaki 9 Masayoshi Kimura 10 Hinano Kurahashi 11 Nagisa Shiota 12 Sosuke Sugaya 13 Tomohito Sugino 14 Kotaro Takebayashi 15 Ryunosuke Chiba 16 Ryoma Terasaka 17 Rio Nakamura 18 Kirara Hazama 19 Rinka Hayami 20 Sumire Hara 21 Yuzuki Fuwa 22 Hiroto Maehara 23 Koki Mimura 24 Takuya Muramatsu 25 Toka Yada 26 Taisei Yoshida 27 Ritsu (Autonomously Intelligence Fixed Artillery) 28 Itona Horibe Class 3-E Classroom Homeroom Teacher: Koro-sensei Assistant Homeroom Teacher: Tadaomi Karasuma Subject Teacher: Irina Jelavić All students are third-year junior high students and assassins. They carry rubber knives and BB pellets to kill Koro-sensei (these two weapons are harmless to humans and only effective against Koro-sensei).