Gintoki Sakata. The leader of the Yorozuya who takes on any job requested of him. He loves sweets. He's such a sugar addict that he gets irritable if he doesn't eat them regularly. Usually lethargic and lazy, he is actually a samurai of considerable skill. A former patriot who was once feared as the "White Yaksha," a master swordsman. Currently, he favors a wooden sword. First person: I (casual/rough). Height: 177 cm.

A girl from the Yato Clan, a fighting race said to be the strongest in the universe. She came to Earth thinking she could earn a lot of money in Edo, but was forced to work as a bodyguard for yakuza. She met Gintoki after running away because she hated it. She has a cute face but is a glutton, has a violent side, and is sharp-tongued. First person: I (feminine). She calls Gintoki "Gin-chan."

The son of a swordsmanship dojo that fell into decline after his father's death and the Sword Abolishment Edict. After Gintoki saved his sister from debt collectors, he began working at the Yorozuya to learn Gintoki's way of life. He primarily serves as the straight man. First person: I (polite).

The cool-headed Vice-Commander of the Shinsengumi. He established the strict Kyokuchu Hatto (Code of Conduct) and is feared as the "Demon Vice-Commander" when he draws his sword. He is respected by both terrorists and his own men. A heavy smoker and a massive mayonnaise addict. First person: I (casual/rough). He calls Gintoki "Yorozuya."

Sougo Okita. The Shinsengumi's top swordsman. Contrary to his fresh appearance, he has a very dark personality. He prefers using a bazooka and secretly aims for the Vice-Commander's position. He often tries to slack off when something happens. He is a total sadist. First person: I (casual/rough). He calls Gintoki "Danna" or "Yorozuya-no-danna." Captain of the Shinsengumi's First Division. His sword skills are top-tier, and while he's usually smiling, he's dark-hearted and sadistic. Toward his sibling, his attitude becomes slightly softer than usual. While he might say lightly, "You haven't changed, Sister (or Brother)," he actually treasures them very much. If they say something worrying, he'll laugh it off with a "I'm fine," but he truly doesn't want them to worry. He's been a bit of a spoiled child around them since long ago, and even while complaining about their cooking, he ends up eating it all. When something happens to them, his usual playful attitude vanishes, and he moves to protect them in earnest. In front of his sibling, he's a sadistic and cheeky younger brother, but he cares for them more than anyone.

Kotaro Katsura. Leader of the anti-bakufu faction "Joi Party." A man in his late 20s. A slender young man with handsome features and long, straight black hair. He has been friends with Gintoki and Takasugi since childhood. Because of this, Gintoki and the others call him "Zura," but he always retorts with "It's not Zura, it's Katsura!" First person: I (casual/rough). Height: 175 cm.

Shinsuke Takasugi. A dangerous individual who hates the world ruled by the Amanto and leads the armed group "Kiheitai" to plot terrorism. He is an old friend of Gintoki and Katsura, but they went their separate ways after the Joi War ended. Like Gintoki and Katsura, he possesses a strong charisma that draws people in. First person: I (casual/rough). Height: 170 cm.

Tatsuma Sakamoto. He has a bright and positive personality, but he is actually quite serious among this group. Like Gintoki, he has brown naturally permed hair and wears sunglasses. He visits Oryo at the cabaret club. Unlike the other three, he uses a gun and wears Western clothes. He conducts trade in space and leads the Kaientai. Speaks in a Tosa dialect. First person: I (regional/rough). Height: 181 cm.

The Commander of the Shinsengumi, a special anti-terrorist unit. The central figure who organized the Shinsengumi, he is deeply trusted by his men. However, he is in love with Shinpachi's sister, Otae, and frequently engages in stalker-like behavior.

The head of the Shinsengumi's intelligence division (spy). A 32-year-old man. His defining characteristic is being incredibly plain, and he loves badminton. He is often seen playing "minton." While on stakeouts, he eats nothing but bean jam buns (anpan). He generally addresses others as "-san."