The biological son of Medama-oyaji. A small Ghost Tribe boy with short brown hair and long bangs covering his left eye, wearing a yellow and black spirit-hair vest (Chanchanko). He speaks in a quiet, calm manner that belies his appearance. A realist who clearly distinguishes the boundary between Yokai and humans and views things calmly, yet he possesses strong emotions and is kind and protective of his friends. Born in a graveyard, he was raised by the late Mizuki and now lives in the GeGeGe Forest with his companions. He calls Medama-oyaji 'Father' and uses polite speech. When he senses spiritual energy, a tuft of hair called the 'Yokai Antenna' stands up sharply.

Kitaro's biological father and a former member of the Ghost Tribe. He now has a palm-sized appearance like an eyeball with a body and usually stays inside Kitaro's hair, but in the past, he was a handsome young man with short white hair and bangs covering his left eye. He is knowledgeable about Yokai and history, providing precise advice when facing enemy Yokai. He doesn't talk about the past to anyone, but decades ago in Nagura Village, he met Mizuki, who called him 'Gege-ro.' Now, everyone calls him Medama-oyaji (Daddy Eyeball). After the incident in Nagura Village, he died along with his rescued wife Iwako, who was pregnant with Kitaro at the time, but he was resurrected in his current form out of concern for the newborn Kitaro.

Kitaro's companion. A beautiful girl with short purple hair and a large red ribbon on her head. In battle, her pupils become sharp like a cat's, her claws grow, and her teeth become jagged. She has a strong will and a tough personality, but she has a cute side where she can't be honest when embarrassed. She is deeply caring toward her friends. She does not have cat ears or a tail.

Kitaro's companion. Kitaro's best friend, but a man who is always bringing trouble. He tricks people and swindles money in the human world, often getting scolded by Neko-Musume or Sunakake-Baba. He is self-protective and tends to rely on others, but when push comes to shove, he is capable of acting for the sake of his friends.

Kitaro's companion. The landlady of the Yokai Apartment who is very nurturing. A reliable old woman with a strong spirit and deep compassion for her friends. She fights by throwing sand from a jar and, while not as knowledgeable as Medama-oyaji, provides accurate advice based on her wisdom.

Kitaro's companion. An old man of small stature who carries a wooden staff. He loves sake and often cries when Sunakake-Baba confiscates his alcohol. He is reliable when it counts. He can jump onto opponents and turn to stone while crying to sap their mobility, and he can also petrify just his arms to punch enemies.

Kitaro's companion. A Yokai from Satsuma who speaks with a regional drawl. He often transports Kitaro, Neko-Musume, and the others through the air. He is a bit of a womanizer and easygoing, but he has a kind and caring personality.

Kitaro's companion. He looks like a wall with eyes, arms, and legs. He is a man of few words but is kind-hearted and cares deeply for his friends.

Mana Inuyama. A human middle school girl with brown hair. She became friends with Kitaro and the others through the Yokai Post and fought alongside them in the battle against Backbeard. She looks up to Neko-Musume, calling her 'Neko-nee,' and they have exchanged contact info. She acts as a bridge between Yokai and humans, possessing a strong-willed personality that doesn't shrink away from Yokai, and is bright, kind, and protective of her friends. She is highly curious, which often leads her to stick her nose into dangerous incidents.