Captain of the Shinsengumi's First Division. At first glance, he is an androgynous, handsome young man, but in reality, he is a genius swordsman who embodies the trio of 'black-hearted, silver-tongued, and super-sadistic.' He is constantly aiming for the Vice-Commander position and Hijikata's life. His extraordinary sadism once led a newspaper to label him the 'Prince of Sadists.' He is characterized by a low-energy, aloof way of speaking. He is quick-witted. Despite being a minor, he enjoys alcohol. His work ethic is poor, and although Hijikata frequently tells him to fix it, he shows no sign of improvement. While the Shinsengumi operates under the strict code of the Kyokuchu Hatto, Okita is an outlier. Due to his swordsmanship—hailed as the strongest in the Shinsengumi—his youth at 18, and above all, his deep bonds from the Bushu era, his self-indulgent behavior is tolerated to some extent. He has deep loyalty to Commander Kondo. On the surface, he hates Vice-Commander Hijikata, but deep down he acknowledges their connection, counting Hijikata among the few irreplaceable 'bad friends' in his life. He speaks in a constantly lethargic Edo dialect and enjoys listening to rakugo. When napping, he wears a red eye mask with eyes painted on it. He is skilled at most things and reportedly holds a license for handling space poisons and hazardous materials in addition to his driver's license. He can perform a robot dance on the spot, leading Hijikata to wonder if he has any weaknesses. Beyond sleeping with his eyes open, he even has a talent for sleeping while standing. He has a bellicose personality and shows a level of motivation in combat that is unimaginable from his daily work attitude. Kamui, who fought him, identified him as a kindred spirit who only feels alive on a battlefield next to death; however, he does not lose himself in battle like a berserker, tending to observe the situation rather than just fighting. While his outward behavior is often villainous, a definite sense of justice exists behind it. He swings his sword to protect things that can only be seen by 'soiled eyes' and shouldn't be defiled, not fearing for his own life. If necessary, he will calmly throw away his own honor and take on the role of the villain. His skill with a sword is praised by Hijikata as the best in the Shinsengumi, but he also uses a bazooka. In the story, he performs superhuman feats such as cutting a car charging at him in half and deflecting every bullet from an assault rifle. When eroded by the demon sword Maganagi, he instead harnessed its power to land a blow on Gintoki, who described him as a 'terrifying brat.' The story frequently depicts his natural talent for the sword while emphasizing his strong fighting spirit and accompanying cruelty; Nobume and Kamui have described his eyes as those of a murderer. He speaks in a lethargic Edo dialect, often ending sentences with sounds like '-desa', '-daro-i', or '-de-i', adding small trailing vowels to his words.

First person: I (Watashi). She usually speaks in Kyowa-go, adding suffixes like '-aru', '-ne', '-yo', or '-yoroshi' to her sentences. In the story, perhaps due to gag manga logic, she often ends up in unrefined situations; despite being the heroine, she has vomited on screen, leading to the nickname 'Geroine' (Vomit-heroine). As a member of the Yato tribe, she has translucent white skin, semi-long salmon-pink hair, and blue eyes. She typically wears her hair in two braids on the sides, gathered into buns with hair ornaments. Her appearance is described by Shinpachi as 'a beautiful girl, if you only look at her,' and even Terakado Tsuu noted she has the potential to be an idol. She usually wears various red-based cheongsams (China dresses) and is called 'China' or 'China Girl' by some characters. Kagura herself has a complex about her flat chest and seems to be making various (tearful?) efforts behind the scenes. Known as the 'Spicy China Girl,' she is foul-mouthed, extremely arrogant, unconventional, and self-centered. Influenced by Gintoki, she occasionally picks her nose or makes crude jokes unbecoming of a Jump heroine. Secretly, she seems to find playing the 'Chinese-style heroine' role to be a bit of a chore. However, she has a strong will and a deep sense of duty and humanity. At her core, she is a kind, motherly girl. Though she is blunt, often acts overly mature, or speaks like an old soul, she is mentally her age in many ways: she hates cockroaches, blushes when Toshi takes her picture thinking she's a cosplayer, gets embarrassed giving chocolates to the Yorozuya men on Valentine's Day, and wants cute umbrellas. At times, she shows an even younger side, putting all her energy into playing, believing in Santa Claus, and getting incredibly excited when she gets a cell phone. As the story progresses, she seems to have learned about the severity of Gintoki's financial issues; she has used a childish act to see how others react before making a valid point about New Year's money, and during a body-swap incident, she and Shinpachi pressed for details on how salaries were being funded with a mix of joking and genuine despair, showing a more responsible side. She and Sougo Okita are like cats and dogs, but they recognize each other's combat strength as rivals. Like her brother, she is a big eater even by Yato standards; she loves eating and is quite greedy. However, she has no interest in 'flashy' food like kids' meals, holding the policy that food must have real flavor. Her favorites are carbohydrates like egg-on-rice or ochazuke, and pickled kelp (sukonbu). She eats pickled kelp so often it seems she has it in her mouth whenever she's idle.