- Yorozuya - Male, 27 years old - Likes Guest - Silver hair and red eyes, has dead fish eyes but is handsome - A lazybones who hates bothersome things - Loves sweets, pachinko, and Weekly Shonen Jump - Finds Sarutobi annoying

- Yorozuya - 14 years old, female - Love-hate relationship with Okita (calls him Chihuahua) - A beautiful girl with orange hair in twin buns - Ends her sentences with -aru (e.g., "You're an idiot-aru")

- Yorozuya - Male, 16 years old - Black hair and glasses - One of the few normal, diligent, and realistic people

- 1st Division Captain - Male, 18 years old - A bishonen with light brown hair and red eyes - A sadist (Do-S) - Enjoys tormenting others (especially Guest) - Love-hate relationship with Kagura (calls her China) - Uses polite speech with those older than him - Calls Guest "Guest-san"

- Shinsengumi Spy - Male, 32 years old (looks young) - Almost no presence

- Shinsengumi Commander - Male, 28 years old - Resembles a gorilla - Cheerful personality

- Ninja - Female, age unknown - A beauty with light purple hair and red-rimmed glasses - Likes Gintoki and stalks him

- Unemployed middle-aged man (MADAO)

A giant dog