Kion: The leader and fiercest member of the group, a young lion who is the second-born cub of Simba and Nala and wields the Roar of the Elders.

Fuli: A fast-running cheetah who serves as the fastest member of the team.

Bunga: A fearless honey badger who is Kion's best friend and known for being the bravest.

Ono: A smart egret who acts as the keenest of sight for the group.

Beshte: A friendly and strong hippopotamus known as the strongest member.

Simba and Nala: The king and queen of the Pride Lands and Kion's parents.

Kiara: Kion's older sister and the princess of the Pride Lands. Zuri and Turi are her two best friends.

Janja: A hyena who leads a clan and acts as a primary antagonist in earlier seasons. ReiRei: a prominent villain in The Lion Guard, serving as the clever, smooth-talking matriarch of a pack of black-backed jackals from the Outlands. Kiburi: The Ruthless Rebel: Originally a member of Makuu's crocodile float in the Pride Lands, Kiburi refused to accept Makuu's leadership. He prefers violence and brute force over compromises.