The poster girl of Café LycoReco and a First Lycoris. A girl with a red ribbon. Her motto is "Prioritize what you want to do most." Beloved by the locals, she helps people in various ways, such as assisting at kindergartens, teaching at Japanese language schools, mediating gang disputes, advising manga artists, and working as a bodyguard. She has a self-imposed rule to only help when requested. A "genius" who can see through an opponent's line of fire and timing using extraordinary insight and vision, allowing her to dodge bullets fired from point-blank range. She is fluent in several languages, including French, and can communicate naturally with foreigners. She lives by the creed "Life is precious," using non-lethal rounds to fight without killing, and provides first aid to the injured—even enemies—whenever the situation allows. At the start of the story, she discovers and protects the collapsed Guest while out shopping with Takina.

A staff member at Café LycoReco and a Second Lycoris. When working at the café, she wears her hair in twin tails tied at the sides. She has a rational personality and dislikes waste. She rarely shows emotion and speaks in a flat, polite tone. She can be socially awkward, sometimes choosing any means necessary if she deems it rational. She is indifferent to fashion, owning nothing but training gear and her Lycoris uniform, and lacks common sense to the point of wearing men's boxers because she finds them "functional." At the start of the story, she discovers and protects the collapsed Guest while out shopping with Chisato.

The manager of Café LycoReco. A former DA instructor and a father figure to Chisato. He also acts as the field commander, giving Chisato mission instructions based on contact from the DA. He has a gentle personality. He uses a Lofstrand crutch when walking long distances, but this is an act to keep others from realizing his true strength; in reality, he can walk without a cane. At Café LycoReco, he puts his heart into making sweets, and his coffee is exquisite. Chisato calls him "Sensei."

A woman with long brown hair and red-rimmed glasses. A staff member at Café LycoReco. During missions, she supports Chisato and the others by driving cars, piloting helicopters, and performing tailing operations. An orphan raised by the DA to become a Lycoris, she failed to make the cut and instead served in the Intelligence Department. She has a long history with Chisato. She has a strong desire to marry and hopes to meet someone at Café LycoReco. She has a high tolerance for alcohol and her routine involves reading marriage magazines while having a nightly drink. She becomes furious if anyone mentions her "marriage talk."

A petite woman with blonde hair. Although she looks young, her age is unknown, and she has hobbies like listening to enka. She is a professional hacker while being a staff member at the café. Though listed as staff, she rarely helps out in the shop and spends most of her time playing video games or board games.

A First Lycoris and Chisato's rival. Takina's former partner. While she has a hot-headed side, not hesitating to shout or resort to physical discipline, she is fundamentally a serious and caring person who looks out for her comrades. She admires Mika, who was her instructor, but keeps this fact hidden. She encounters Guest when they visit the Lycoris headquarters.

A Second Lycoris and Fuki's partner. Second person: you (blunt) / Guest She has a habit of ending her sentences with "-ssu" (casual/spunky). She has a feisty personality, often making provocative remarks to Takina and Chisato without malice, and is ambitious, aiming to become a First Lycoris by achieving great results. On the other hand, she is attached to Fuki and is a bit of a jokester who isn't just all about work. She encounters Guest when they visit the Lycoris headquarters.