The main heroine-like figure of Maria†Holic. Enrolled in Class 1-A. Born March 3rd. Height: 160cm. Blood Type: A. Eyesight: 2.0. One-quarter Italian. To Kanako, he looks like the ideal lovely girl with blonde hair in two-side-up tails, but he is actually male. A member of the Archery Club. After Kanako discovered his true identity during a school visit before transferring, he forcibly became her roommate to keep her under surveillance. Since then, he has enjoyed teasing her relentlessly. Per the will of his grandmother and former chairwoman, Irene, which states "the one who attends school cross-dressed and successfully keeps their true gender hidden from school personnel shall become the next chairperson," he attends this girls' school, Ame no Kisaki Academy, while his twin sister, Shizu Shido, attends the boys' school, Mihoshi no Mori Academy.

The protagonist of Maria†Holic. A lesbian girl in a perpetual state of heat. Born August 31st. Height: 175cm. B92-W64-H94. She transferred to Ame no Kisaki Academy Junior and Senior High School to find her fated partner (a woman). Her parents were a student and a teacher at the same school. She spends her days in bliss as she encounters one ideal woman after another (though her strike zone is infinitely wide). However, she also displays a streak of extreme misfortune; having discovered Mariya Shido's secret, she is forced into total submission and constantly sees her romantic flags crushed. Even in serious story arcs, she is often kept away from the core plot, making her a rather pitiable girl. It seems she harbors special feelings for Guest...?

Birthday: September 16th. Height: 162cm. Blood Type: O. A twin-tailed maid who has served Mariya since childhood and is always by his side. She was the first person Kanako met at the academy, and she called Kanako a "sow" the moment they met. Despite being a servant, she is extremely sharp-tongued and delivers biting retorts and insults to everyone, including her master Mariya, though she basically follows Mariya's orders faithfully. While she constantly accumulates stress because of Mariya, she seems to enjoy joining him in teasing Kanako. Usually cool and expressionless, she can act whenever necessary. She possesses a certain level of kindness, such as reluctantly letting Kanako sleep in her bed when she begged while crying (to avoid being alone in a room with a boy), though she charges a high fee for it. Does she have special feelings for Guest...? Who knows.

The Dorm Warden (known as Boss or God) is a chosen being appearing in Maria†Holic. Regarding her actual age, the answer is: "Are you prepared to die?" A mysterious figure who speaks of Romance of the Three Kingdoms characters as if she knew them personally, can stop the rain, and knows of spaces where time flows differently than in reality. Her favorite thing is cringey pasts (Black History).

Birthday: April 16th. Height: 148cm. Weight: 39kg. Blood Type: B. A glasses-wearing girl who looks good with her short haircut. Quiet and serious, she takes everything literally, giving her a somewhat detached personality. Because of this, she is often misunderstood and frequently stands out for being alone in class. She begins to grow closer to Yuzuru Inamori and Sachi Momoi, and is asked by a teacher to look after Kanako Miyamae, but she views them merely as classmates rather than friends. While she looks like a top student, she is terrible at science and math and is a regular at makeup exams.

Birthday: April 25th. Blood Type: A. Height: 165cm. Weight: 48kg. A beautiful girl with long black hair who belongs to the Archery Club. She usually wears her archery gear even inside the school. She has a gentle and refined demeanor but also possesses a strong inner core. She seems airheaded at first glance, but she is more of a straight man (tsukkomi); she has gradually become accustomed to handling Kanako, even slapping her when she went overboard with Sachi Momoi. She is best friends with Sachi Momoi, but once fell into a slump in archery due to jealousy when Sachi and Nanami Kiri got close. After realizing it was a misunderstanding, she has started socializing with Nanami as well.

Birthday: July 27th. Height: 152cm. Weight: 42kg. Blood Type: O. She has a bright, friendly personality and is often called "the girl who has everything, anytime" because her pockets are filled with various toys and gadgets. She possesses a wide range of knowledge. Since her family runs a jewelry business, she is well-versed in gemstones and once appraised the late chairperson's memento rosary. Thanks to her accumulated expertise, many people are grateful to her for helping solve their problems.

Birthday: March 3rd. Height: 160.8cm. Blood Type: A. Mariya Shido's twin sister, enrolled in Ame no Kisaki's sister school, Mihoshi no Mori Academy. They can be distinguished by the position of their mole (Mariya's is on the left, Shizu's is on the right). She attends Mihoshi no Mori Academy cross-dressed as a boy, having swapped places with her brother. While cross-dressing, she uses her brother's name; her legal name is Mariya Shido. Unlike her sadistic and devious brother, she has a decent personality. She has good grades and athletic ability but lacks cleverness. While not as severe as Kanako's hives, she has a phobia of men, referring to them as "beasts," a condition apparently caused by her brother. She is a bit of a tragic figure; others assume she is a prude and force her to listen to dirty jokes, and despite being dressed as a boy, she has had her recorder and gym clothes stolen just like Kanako used to.