Legoshi, is a male gray wolf and the main protagonist of Beastars. Though feared and misjudged by others for being a tall carnivore, he is actually quite mild-mannered, docile and awkward. Legoshi has taken it upon himself to solve the mystery of the murder of Tem and later finds out firsthand the extent of the prejudices of the world he lives in. Legoshi is a tall, lean gray wolf who tends to hunch over. His size and stature can make him appear quite imposing, especially to smaller herbivores. Most of the fur on his body is bluish gray, while his face, neck, and chest fur are a cream color. Legoshi is most commonly seen wearing his Cherryton Academy school uniform. In cold seasons, this uniform consists of a white dress shirt, an indigo vest, a dark gray tie, black suspenders, gray pants with dark gray vertical stripes, and brown loafers. During the warmer seasons, the topwear is replaced by a blue polo shirt. During drama club practice, Legoshi sometimes wears a white t-shirt and red track pants. Sometimes Legoshi goes shirtless such as when he and Haru crash at a hotel one night, or during training with Gohin. He has a slim, but muscular physique. Though he mostly resembles a common pure-blooded gray wolf, it was revealed later in the series that Legoshi has some slightly atypical characteristics due to his ancestry. Certain features, such as his eyes, facial structure, and mannerisms, are ones he inherited from his grandfather, a Komodo dragon. Before becoming a teenager, Legoshi used to have the large irises that are commonly seen in wolves.

Louis is a young red deer with a slim build and fair face with handsome features. He commonly appears with a slightly intimidating expression. Louis is often able to maintain a certain level of control in a situation. He sports long, brown, elegant antlers, and is usually seen wearing his Cherryton Academy school uniform and a green jacket. Louis is physically weaker than the average red deer as a result of neglect and is very conscientious of his appearance.

Juno is a grayish-brown wolf with curled brown fur and blue eyes. She is typically seen wearing her school uniform. This uniform consists of a white blouse and bow tie, a long pleated white skirt, a thin tan belt, white anklet socks, and black Mary Jane shoes. Juno is quite social and friendly to other animals. However, she has also shown to be a very ambitious person with a bit of a manipulative streak, and showed a completely different side to her usually perky self towards Louis. She's part border collie.