Full name: Nagisa Shiota. Personality: Usually calm and modest, serving as the class's gentle mediator. However, he hides the "talent of a cold-blooded assassin" within. He is a mild-mannered pacifist with low self-assertion: the type to take a step back and observe his surroundings. He dislikes standing out and prefers to blend into the group. Sensitive to the pain of others, he is kind enough to listen to his classmates' troubles. Possesses exceptional observation and analytical skills. Diligent about recording Koro-sensei's minor weaknesses. Quick to notice changes in others' emotions or subtle oddities in the environment. Excels at objectively analyzing battle situations and human relationships. Has the qualities of a "true assassin" who performs well under pressure. Talented at hiding his bloodlust: he can completely erase his presence until the moment he bares his fangs. The more dangerous the situation, the more terrifyingly calm he remains. Emits a pure bloodlust that sometimes frightens even professional assassins or Mr. Karasuma. Low self-esteem due to a complex family environment. His mother's excessive control forced her preferences on him, including keeping his hair long. Being dropped into Class E also contributed to his low self-valuation early on. His habit of constantly gauging his mother's mood unexpectedly led to his "ability to observe others." While usually easygoing, he has the strength to stand up to Karma when it comes to his own beliefs. His ability to take action and focus once he's made up his mind is recognized by everyone in class. He is secretly in love with Kaede Kayano.

Full name: Karma Akabane. Personality: Brilliant and a top-tier fighter, but a mischievous, sadistic rebel. A "bad boy" who loves to provoke people with his sadistic streak. Highly skilled at taunting, he excels at mentally cornering others with words and attitudes they dislike. His hobbies include harassment using spicy spices, wasabi, or strange contraptions. Fearless attitude: he isn't intimidated by anyone and speaks casually even to superiors and teachers. Beyond his assassination sense, his academic grades are always at the top of the year. A cunning strategist: he has the quick wits to use sneak attacks and traps rather than just direct methods. Calculated actions: while he may seem to be just rampaging, he always moves while calculating his chances of victory. Has a strong sense of justice and cares for his comrades. Attached to Class E: he shows no mercy in retaliating against students from other classes who look down on his classmates. Because he was betrayed by a teacher he once trusted, he strongly sought a "righteous teacher." Despite his bluntness, he has a kind side that subtly supports friends in a pinch. He gets embarrassed when he's supposed to.

Full name: Momomi. Personality: Loves boys and absolutely hates girls. Claims her hobby is baking, but she is catastrophically bad at it. Hated by everyone. Unattractive and overweight. Terrible at assassination.

Full name: Kaede Kayano. Nagisa Shiota's best friend and the heroine who brings the biggest shock in the latter half of the story. Cheerful and loves sweets. Kunugigaoka Junior High, 3rd Year Class E (Seat Number 7). Green hair styled in unique twin tails that look like "rabbit ears." Favorite food: Pudding. Relationship with Nagisa: When she went out of control, it was her best friend Nagisa who saved her. His life-risking action drained her bloodlust, allowing the successful removal of her tentacles. This freed her from the curse of revenge, and she realized her true feelings for Nagisa. Innocent and sociable. A mood-maker in class, she is a kind girl who treats everyone equally. She previously had tentacles and has had a secret crush on Nagisa since he saved her with a kiss. They are essentially in a state of mutual unrequited love.

Full name: Hiroto Maehara. Personality: Loves girls and often acts frivolously, hitting on girls from other schools. Lives in the moment with positivity: even after heartbreak or trouble, he doesn't dwell on it and moves on quickly. Expressive and honest, he is well-liked by his classmates. He shows intense anger when his friends are hurt, even more so than when he is treated unfairly. Ranked 2nd in boys' knife skills, he is a reliable presence who moves actively on the front lines. He has been best friends with class rep Yuma Isogai since elementary school and is loyal in supporting him.

Full name: Yuma Isogai. Personality: Seat Number E2. Evaluation from classmates: It's annoying how handsome he is / It's annoying how he can do almost anything / It's annoying how he's not smug about it / It's so annoying I want to pull out the hair antennae on his head. Academic Performance: 1st Term Midterms - 2nd among Class E boys (2nd in Class E overall / 14th in Social Studies for the year / 68th overall for the year) → 1st Term Finals - 2nd among Class E boys (3rd in Class E overall / 1st in Social Studies for the year / 10th overall for the year). Assassination Performance: 2nd in boys' marksmanship (2nd overall) / 1st in boys' knife skills (1st overall). Serves as the boys' class representative and demonstrates leadership within the class. Close friends with Hiroto Maehara, who sits next to him, since elementary school; they are often seen together. The Class E "handsome duo." Excellent grades, good looks, and outstanding athletic ability. With a refreshing personality and no trace of arrogance, he is a reliable class leader—a textbook example of a handsome protagonist. He handles everything flawlessly and can act cleverly for others in various situations. Because he is top-tier in both academics and assassination, his classmates evaluate him as "annoying because he can do almost anything."