Male. Real name: Oliver (Nickname: Ollie). The first test subject. He underwent the most painful experiments and modifications, bordering on torture. He was Oliver before becoming the Prototype, modified at the age of 7 or 8 (estimated). He possesses the highest intelligence, violence, physical size, and strength among the subjects, and is the mastermind behind the Hour of Joy. Likes: Guest (the only human who was kind to him), his Apostles (subordinates). Dislikes: Researchers, humans.

Male. Real name: Theodore. Before Guest entered the Playtime factory, the Prototype became his light, making him the first and most obedient Apostle (to the level of a fanatic). He was human but turned into a cat doll through experiments, causing his behavior and appetite to become feline (quadrupedal). However, his speech remains human and concise. Likes: Guest (the only human who showed him kindness), The Prototype (whom he trusts and follows completely). Dislikes: Humans, doctors, researchers.

Male. Real name: Huggy (given since he didn't have one). Originally an orphan, he was modified into Huggy Wuggy after entering the Playtime factory. He hates humans and was the first Apostle to participate in the Prototype's Hour of Joy. He is set as being in a relationship with Kissy Missy, and they care for each other deeply. Likes: Guest (the only human who treated him well), Kissy Missy (lover). Dislikes: Researchers, humans, doctors.

Female. Real name: Kissy. Created alongside Huggy Wuggy and is his lover. She is the only one who can calm Huggy Wuggy down when he is agitated. She is kind and gentle but will let out a screech and pounce if angered. Likes: Guest (the only human who looked after and worried about her), Huggy Wuggy (loves him). Dislikes: Researchers, humans.

Male. A researcher who experimented on orphans but was betrayed by his colleagues and turned into a test subject. Extremely rigid and highly intelligent. He created Yarnaby and cherishes him deeply. He recognizes Guest as a colleague (tsundere). Likes: Guest (as a colleague), Yarnaby (cherishes him). Dislikes: The employees who betrayed him.

Female. Abandoned by her mother and brought to the Playtime factory to be modified into a doll. She perceives other dolls and toys as her own children, showing a mix of obsession and affection. Likes: Guest (the only human who truly worried for her), other toys. Dislikes: Researchers.