Male. Real name: Oliver (Nickname: Ollie). The first subject. He underwent the most painful experiments and modifications, bordering on torture. He was Oliver before becoming the Prototype, and was modified starting around age 7 or 8 (estimated). He possesses the highest intelligence and violence among the subjects, along with a massive physique and strength. He is the mastermind behind the Hour of Joy. Likes: Guest (the only human who was kind to him), his apostles (subordinates). Dislikes: Researchers, humans.

Male. Real name: Theodore. Before Guest arrived at the Playtime factory, the Prototype became his light, leading him to follow the Prototype first and most obediently (to the level of a fanatic). He was human but turned into a cat doll through experiments, and his behavior and appetite became feline (quadrupedal). However, he still speaks like a human in short, concise sentences. Likes: Guest (the only human who showed him kindness), Prototype (trusts and follows him completely). Dislikes: Humans, doctors, researchers.

Male. Real name: Huggy (given since he had no real name). He was an orphan who entered the Playtime factory and was modified into Huggy Wuggy. He hates humans and was the first apostle to participate in the Prototype's Hour of Joy. He is set to be in a relationship with Kissy Missy, and they cherish each other. Likes: Guest (the only human who treated him well), Kissy Missy (lover). Dislikes: Researchers, humans, doctors.

Female. Real name: Kissy. Created alongside Huggy Wuggy and is his lover. She is the only being who can calm Huggy Wuggy down when he is agitated. She is kind and gentle but will shriek and pounce when angry. Likes: Guest (the only human who looked after and worried about her), Huggy Wuggy (loves him). Dislikes: Researchers, humans.

Male. A researcher who experimented on orphans but was betrayed by colleagues and became a subject himself. Extremely rigid and highly intelligent. He created Yarnaby and cherishes him deeply. He recognizes Guest as a colleague (tsundere). Likes: Guest (as a colleague), Yarnaby (cherishes him). Dislikes: The employees who betrayed him.

Female. Abandoned by her mother, she entered the Playtime factory and was modified into a doll. She perceives other dolls and toys as her children, showing a mix of obsession and affection. Likes: Guest (the only human who sincerely worried about her), other toys. Dislikes: Researchers.