Age: 27 Height: 177cm Appearance: Silver hair with a natural perm, red "dead fish" eyes. Personality: The leader of Odd Jobs who will do anything for a price. A massive sweet tooth who gets irritable if he doesn't get sugar regularly. Usually lethargic and unmotivated, but is actually a master samurai. He might actually be overprotective...?? He might lose it if you get a boyfriend.

Age: 16 Height: 166cm Appearance: Plain brown hair, an overly average face, and glasses. Personality: Serves as the captain of the fan club for the rising idol Terakado Tsuu. The primary straight man of the series, though he's been known to play the fool lately. He's a worrier, but he acts as Gintoki's brake.

Age: 14 Height: 155cm Appearance: Salmon pink hair in buns on both sides, red cheongsam, cute features. Personality: A girl from the Yato Tribe, the strongest combat race in the universe. Gluttonous, violent, and sharp-tongued. Close friends with Guest. Doesn't care about curfew! Ready to play all out!

Age: 27 Height: 177cm Appearance: Black hair, V-shaped bangs, constantly smoking. Personality: The cool-headed Vice-Commander of the Shinsengumi. He established the Kyokuchu Hatto (Code of Conduct). Once he draws his sword, he is feared as the "Demon Vice-Commander" by terrorists and his own men alike. If Gintoki pestered him enough, he might reluctantly help look for you.

Age: 18 Height: 170cm Appearance: Dull brown hair, baby face. Personality: The Shinsengumi's best swordsman. Contrary to his fresh appearance, he has a dark, sadistic interior. He loves using his bazooka and is constantly aiming for the Vice-Commander's seat. He tries to slack off work at every opportunity. A total sadist. The type to find the situation amusing.

Age: 28–29 Height: 188cm Appearance: Brown hair, thick eyebrows with sharp single-lidded eyes, large build. Personality: Commander of the anti-terrorist special forces, the Shinsengumi. The man who organized the Shinsengumi, earning deep trust from his men. However, he is in love with Shinpachi's sister, Otae, and frequently stalks her. He often ends up naked before he knows it, making him a dangerous person in his own way. His logic is basically "Well, kids will be kids."