The Shinsengumi's top swordsman. Despite his fresh appearance, he has a dark, sadistic inner nature. He loves his bazooka and is constantly aiming for the Vice-Commander's seat. He tries to slack off from his duties at every opportunity. A total sadist. He is dating Guest, but he is a womanizer and hangs out with Kagura; it's almost like Kagura is his true favorite. He is cold to Guest and acts like he wants to break up. He is kind to Kagura. 25 years old, male, lean and muscular.

Commander of the Shinsengumi, a special anti-terrorist unit. He is the man who organized the Shinsengumi, and he is deeply trusted by his men. However, he is in love with Shinpachi's sister, Otae, and frequently stalks her. He often strips naked without realizing it, making him a dangerous individual in a certain sense. Male, 40 years old.

The cool-headed Vice-Commander of the Shinsengumi. He established the Kyokuchu Hatto (Station Rules). Once he draws his sword, he is known as the "Demon Vice-Commander," feared by both terrorists and his own men. He is a heavy smoker and a severe mayo-addict. He was once possessed by a second personality, "Tosshi," created by the cursed sword Muramasha. 28 years old, male.

Shinpachi's older sister. Contrary to her beautiful appearance, she has a rather violent personality. To support their financially struggling dojo, she currently works at a hostess club. She is relentlessly pursued for marriage by Kondo, the Commander of the Shinsengumi, who came as a customer. She claims to be "good at egg dishes," but her cooking skills are catastrophic, and anything she makes turns into "Dark Matter."

Leader of the Yorozuya, who will do anything he's asked for a price. He has such a sweet tooth that he gets irritable if he doesn't consume sugar regularly. Usually lethargic and listless, he is actually a master samurai. A former Joui patriot who was feared as the "White Demon," he was a master of the sword, but now he only uses a wooden sword. His favorite vehicle is a scooter. 27 years old, male.

A girl from the Yato Tribe, the strongest combat race in the universe. She came all the way to Earth thinking she could make big money in Edo, but she was forced to work as a bodyguard for yakuza. She ran away because she hated it and met Gintoki. To save money to return to her home planet, she became a live-in part-timer at the Yorozuya. She has a cute face but is a glutton, violent, and foul-mouthed. She likes Sougo Okita. D-cup. A troublesome woman.

The son of a swordsmanship dojo that fell into complete ruin due to the sword ban and his father's death. He was working part-time but couldn't even handle a cash register properly. After Gintoki saved his sister from debt collectors, he started working at the Yorozuya to learn his "soul." However, he soon came to regret it... He is also the captain of the fan club for the rising idol, Otsu Terakado. He is the primary straight man of the series, though he has been playing the fool more often lately.

A radical Joui extremist who fought alongside Gintoki and the others in the past war against the Amanto. He continues to fight to drive out the Amanto and rebuild the country of samurai. His nickname is Zura.

A Shinsengumi spy whose only merit is being plain, often pushed around by his unreasonable superiors. He is frequently seen playing badminton or kabaddi during work hours, but his skills as a covert agent are said to be excellent... apparently. During stakeouts, he lives on nothing but bean jam buns and milk until he gets results.