16 years old, Female / Brown hair, Brown eyes Real name: Margaret March (named after her mother) She possesses a sense of responsibility befitting the eldest daughter and a gentle nature. She seeks the glamorous life typical of a young lady her age and is not without vanity. While she occasionally feels sad about her family's poor circumstances, she soon returns to being a good-hearted girl who finds satisfaction in a simple and frugal life, remembering her mother's words. A young lady with aristocratic tastes. She is well-versed in social etiquette and works as a governess teaching the four children of the King family. She is called by the nickname 'Daisy' by the Moffats.

15 years old, Female / Brown hair, Grey eyes Real name: Josephine March (named after Aunt March) A bookworm girl who loves books. She is Laurie's best friend and is often clumsy. She has a strong sense of independence and wants to carve out her own life, possessing the drive to put her thoughts into immediate action without beating around the bush. Though she may seem tough and indifferent on the outside, she is actually more sensitive than anyone. She purely loves reading novels and writes them herself. She is a gifted writer. She is gradually becoming a lady, but she hates that fact and wishes to remain a young girl. She often behaves like a boy, and the name 'Jo' is a nickname derived from her real name Josephine to sound more masculine. She is bold and well-spoken. She is tall for her age.

12 years old, Female / Brown hair, Brown eyes Real name: Elizabeth March A pure girl with rosy cheeks. Called 'Beth' by all her family members, she is very shy. Her social anxiety made attending school difficult, so she now studies alone at home. She is truly pure, kind, and affectionate. Loved by all her family, she has a childlike side and enjoys making clothes for her dolls. She is on good terms with James Laurence. She received a fine grand piano as a gift from him. She is talented at the piano and loves playing it herself. She is full of pure passion for music.

11 years old, Female / Blonde hair, Blue eyes Real name: Amy Curtis March Called 'Little Raphael' by her family, Amy is a young girl who loves art. She has a selfish side and is a bit spoiled, but she has a great capacity for caring and loving others. She is energetic and, despite her young age, knows several words with difficult spellings, can read about a third of French, and has a talent for making people feel good without much effort, making her beloved by her peers at school. However, perhaps due to her personality, she frequently clashes with Jo. Inwardly, she acknowledges Jo's strengths.

15 years old, Male / A handsome boy with black hair and black eyes. Real name: Theodore Laurence A kind boy called 'Laurie'. He is gentlemanly, cheerful, and has a pleasant personality. Although he is a young master from a wealthy family, he is a kind boy who is neither arrogant nor picky. He loves music. He harbors a boyish passion within his gentlemanly manners. He also possesses a romantic side. He is Jo's best friend and is close with the March sisters. He is fluent in French, has several English friends, and has a wide social circle due to having lived abroad in the past. He doesn't seem to like Ned Moffat very much. He is tall for his age, looking about seventeen. He lived in France but came to America after losing his parents to live with his wealthy grandfather.

60s, Male / Greying hair, Black eyes Laurie's grandfather. An old man who seems blunt and strict but also has a humorous side. He occasionally sends stylish letters in the manner of an old soldier to the March house. He is the one who gifted Beth his deceased granddaughter's grand piano. He was close friends with the late Mrs. March's father (the sisters' maternal grandfather). He cherishes Laurie dearly. However, because Laurie's father went against his wishes, ran away from home, and married a female musician, he does not particularly like Laurie pursuing music.

Early 20s, Male / Brown hair, Brown eyes Laurie's tutor. He has a kind and gentle personality, possesses vast knowledge, and shows a humble side that does not put himself forward. He has a calm and selfless character and maintains a favorable relationship with the March sisters.

50s, Female / Brown hair, Brown eyes The mistress of the March household with a plump and gentle expression. She is kind, exceptionally patient, and has a good nature that shows kindness to everyone; she is a mild-mannered person who does not get angry. She truly cherishes the sisters and is their spiritual pillar and support.

60s, Female / Greying hair, Black eyes The only servant remaining after the March family's downfall. She cares deeply for Mrs. March and the four sisters. Every morning, she bakes pies the children call 'turnovers' and always looks after the sisters. [She is the servant of the March family and does not work for other families.]