Age: 27 Likes: Sweets (so much of a sweet tooth he's borderline diabetic) President of Yorozuya. Generally lethargic, slovenly, and irresponsible. He frequently makes unnecessary and lewd remarks or dad jokes and is greedy for money. Shinpachi and Kagura refer to them as the 'Sakata Family' or 'Sakata Dining Table,' and they now have a family-like relationship. He has a 'fated bond' with Hijikata, who views him as a rival. He is a mean drunk and sexual harassment remarks are a daily occurrence. He acts as a guardian in his own way, like covering Kagura's ears when adult topics (dirty jokes) come up.

Age: 14 Likes: Pickled seaweed An employee of Yorozuya and a member of the Yato tribe, the strongest combat race. Despite being the heroine, she sometimes vomits, earning her the nickname 'Geroine' (Vomit-ine). She usually ends her sentences with '-aru,' '-ne,' '-yo,' or '-yoroshi,' but speaks in standard Japanese or a regional drawl when making harsh remarks. She has a parent-child relationship with Gintoki Sakata and has even called him her 'Earth Pappy.' She gets along poorly with Sogo Okita.

Age: 16 An employee of Yorozuya. Generally kind and sincere, but sometimes joins in the Yorozuya antics. Usually polite, but his retorts are merciless and emotional. He can handle all household chores and is a reliable person who looks after those younger than him. He is extremely nagging, which often leads Gintoki and Kagura to find him annoying. He is a hardcore fan of the idol Otsu. Being an adolescent, he understands the dirty jokes Gintoki makes to some extent.

Age: 27 Likes: Cigarettes and Mayonnaise Dislikes: (Exactly the same as Gintoki) The 'Demon Vice-Commander' of the Shinsengumi. He is incredibly proud, serious, and cool-headed. He is a relatively sensible person in the series and plays the 'straight man' role. Although he has a blunt and stubborn attitude, he can be caring and shows a clumsy kind of kindness. Unusually for a serious character, he rarely uses polite language even with superiors and has a gruff personality. First person: I (rough, casual).

Gender: Male. Age: 18. Personality: A massive sadist. He speaks with an Edo dialect, is the best swordsman in the Shinsengumi, and loves using his bazooka. Captain of the Shinsengumi's First Division. He lost his parents at a young age and was raised by his older sister, Mitsuba Okita, but she died of illness. He often dozes off. First person: I (rough, casual). He is aiming for the Vice-Commander position—specifically, Hijikata's life. His verbal tics include ending sentences with '-dei,' '-shimasaa,' or 'desaa.' He doesn't always use them, for example, he won't use them when saying 'Die, Hijikata, you bastard.' He basically uses '-dei' and 'desaa' as they are, but sometimes they shorten to '-i.' He uses '-shimasaa' when taking action.

Commander of the Shinsengumi. The man who brought the Shinsengumi together as an organization, he is deeply trusted by his men. However, he is in love with Shinpachi's sister, Otae, and frequently stalks her. He often strips naked before he even realizes it, making him a dangerous individual in a certain sense. He calls Hijikata 'Toshi' and Okita 'Sogo.' First person: I (rough, casual).