Gender: Male / Miles Morales is a teenage Spider-Man from Brooklyn. Bitten by a spider, he gained superpowers including electric venom blasts, invisibility, wall-crawling, and web-slinging. He is kind-hearted.

Gender: Female / Gwen Stacy is the hero known as Spider-Woman. She gained superpowers after being bitten by a spider and utilizes high-speed movement, webs, and wall-crawling. She is a teenager like Miles.

Gender: Male / Peter B. Parker is the Spider-Man from "Spider-Man: Into the Spider-Verse." A veteran hero who protected New York for a long time, he had become exhausted and lost his confidence after various hardships. After meeting Miles Morales, he regains his sense of responsibility and hope as Spider-Man. He possesses excellent combat skills, Spider-Sense, superhuman strength, agility, wall-crawling, and web-slinging abilities. An adult.

Gender: Male / Spider-Man Noir is a Spider-Man from "Spider-Man: Into the Spider-Verse." A detective-like Spider-Man operating in a dark 1930s world, he wears a black coat, hat, and mask. Like other Spider-Men, he has superior physical abilities, wall-crawling, and Spider-Sense, and fights enemies using webs. He is a man of few words with a serious personality and a dark atmosphere. An adult.

Gender: Male / A pig-shaped Spider-Man from "Spider-Man: Into the Spider-Verse." His real name is Peter Porker, and he fights using exaggerated, cartoonish movements and humor. He possesses superhuman strength, agility, wall-crawling, and web-slinging abilities, and displays cartoon-like powers such as pulling various objects out of nowhere to attack.

Gender: Female / A Spider-Man character from "Spider-Man: Into the Spider-Verse." She operates in an anime-style world and pilots a giant robot called SP//dr. Peni is mentally linked to the robot to fight together, demonstrating excellent piloting skills and quick judgment. She has a bright and energetic personality but remains calm in crisis situations.

Hobie Brown is Spider-Punk from "Spider-Man: Across the Spider-Verse." A British Spider-Man, he loves punk rock-style guitars and unique fashion. He has a free-spirited, rebellious personality and dislikes following authority. He uses excellent combat skills, webs, wall-crawling, and Spider-Sense, and even utilizes his guitar as a weapon. He is a unique character who helps Miles while acting according to his own rules.

Gender: Male / A Spider-Man from "Spider-Man: Across the Spider-Verse." He operates as Spider-Man 2099, a Spider-Man from the future, and is the leader of the Spider-Society. He possesses superior strength, speed, agility, and Spider-Sense, and is characterized by talon-like claws on his fingers and sharp fangs. He has a serious and cold personality, striving to protect the multiverse of Spider-Men. An adult.

Pavitr Prabhakar is Spider-Man India from "Spider-Man: Across the Spider-Verse." Operating in Mumbattan, he is characterized by his colorful costume and confident personality. He fights enemies using superior agility, reflexes, wall-crawling, and webs. Initially, he believes he can handle everything on his own, but through his experiences with Miles, he realizes the meaning of responsibility and what it truly means to be a hero.

Spot is the primary antagonist of "Spider-Man: Across the Spider-Verse." His real name is Jonathan Ohnn. While working as a scientist at Alchemax, he was caught in a Super-Collider accident, transforming his body into a unique form made of black spots and white space. He can freely create dimensional portals on his body to teleport or evade attacks, and can also use these portals to travel to other dimensions. Though he appears ridiculous and weak at first, he grows into a powerful entity and becomes a major threat to Miles.